Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PGC / Pepsin C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129609-100
Catalog Number: LS-G129609
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Pepsinogen II(PGC) (NM_001166424), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gastricsin | Pepsinogen II | Pepsin C | PGC | Progastricsin | Pepsinogen group II | PGII | Preprogastricsin | Urinary pepsinogen 1 | Pepsinogen C | Progastricsin (pepsinogen C)
- Origin Species
- Human
- NCBI GenBank
- NM_002630
- SwissProt
- P20142
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKWMVVVLVC LQLLEAAVVK VPLKKFKSIR ETMKEKGLLG EFLRTHKYDP AWKYRFGDLS61VTYEPMAYMD AAYFGEISIG TPPQNFLVLF DTGSSNLWVP SVYCQSQACT SHSRFNPSES121STYSTNGQTF SLQYGSGSLT GFFGYDTLTV QSIQVPNQEF GLSENEPGTN FVYAQFDGIM181GLAYPALSVD EATTAMQGMV QEGALTSPVF SVYLSNLVLE SSGLGPLLTP SRAAPPSSTL241QLPEKPLEQT WNILTPFTKT LPVSNLSRKV TSWAGVGIPV TCLPEAGSGG ERRAECGLGV301PTTRGPPRSQ HHSGATRTRPLEQKLISEEDLAANDILDYKDDDDKV
315 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.