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PEX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PEX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G125205-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEX5 (NM_001131023), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PBD2A | PBD2B | Peroxisome receptor 1 | PTS1 receptor | PTS1-BP | PTS1R | PXR1 | Peroxin-5 | PEX5
Origin Species
Human
NCBI GenBank
NM_000319
SwissProt
P50542

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMRELVEAE CGGANPLMKL AGHFTQDKAL RQEGLRPGPW PPGAPASEAV SVLEVESPGA
61ASEAASKPLG VASEDELVAE FLQDQNAPLV SRAPQTFKMD DLLAEMQQIE QSNFRQAPQR
121APGVADLALS ENWAQEFLAA GDAVDVTQDY NETDWSQEFI SEVTDPLSVS PARWAEEYLE
181QSEEKLWLGE PEGTATDRWY DEYHPEEDLQ HTASDFVAKV DDPKLANSEF LKFVRQIGEG
241QVSLESGAGS GRAQAEQWAA EFIQQQGTSD AWVDQFTRPV NTSALDMEFE RAKSAIESDV
301DFWDKLQAEL EEMAKRDAEA HPWLSDYDDL TSATYDKGYQ FEEENPLRDH PQPFEEGLRR
361LQEGDLPNAV LLFEAAVQQD PKHMEAWQYL GTTQAENEQE LLAISALRRC LELKPDNQTA
421LMALAVSFTN ESLQRQACET LRDWLRYTPA YAHLVTPAEE GAGGAGLGPS KRILGSLLSD
481SLFLEVKELF LAAVRLDPTS IDPDVQCGLG VLFNLSGEYD KAVDCFTAAL SVRPNDYLLW
541NKLGATLANG NQSEEAVAAY RRALELQPGY IRSRYNLGIS CINLGAHREA VEHFLEALNM
601QRKSRGPRGE GGAMSENIWS TLRLALSMLG QSDAYGAADA RDLSTLLTMF GLPQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

654 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.