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PER3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PER3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112491-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PER3 (NM_016831), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GIG13 | HPER3 | Period circadian protein 3 | PER3 | Period 3 | Period (Drosophila) homolog 3 | Period circadian clock 3 | Period homolog 3 (Drosophila) | Growth-inhibiting protein 13
Origin Species
Human
NCBI GenBank
NM_016831
SwissProt
P56645

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRGEAPGPG RRGAKDEALG EESGERWSPE FHLQRKLADS SHSEQQDRNR VSEELIMVVQ
61EMKKYFPSER RNKPSTLDAL NYALRCVHSV QANSEFFQIL SQNGAPQADV SMYSLEELAT
121IASEHTSKNT DTFVAVFSFL SGRLVHISEQ AALILNRKKD VLASSHFVDL LAPQDMRVFY
181AHTARAQLPF WNNWTQRAAR YECAPVKPFF CRIRGGEDRK QEKCHSPFRI IPYLIHVHHP
241AQPELESEPC CLTVVEKIHS GYEAPRIPVN KRIFTTTHTP GCVFLEVDEK AVPLLGYLPQ
301DLIGTSILSY LHPEDRSLMV AIHQKVLKYA GHPPFEHSPI RFCTQNGDYI ILDSSWSSFV
361NPWSRKISFI IGRHKVRTSP LNEDVFATKI KKMNDNDKDI TELQEQIYKL LLQPVHVSVS
421SGYGSLGSSG SQEQLVSIAS SSEASGHRVE ETKAEQMTLQ QVYASVNKIK NLGQQLYIES
481MTKSSFKPVT GTRTEPNGGG ECKTFTSFHQ TLKNNSVYTE PCEDLRNDEH SPSYQQINCI
541DSVIRYLKSY NIPALKRKCI SCTNTTSSSS EEDKQNHKAD DVQALQAGLQ IPAIPKSEMP
601TNGRSIDTGG GAPQILSTAM LSLGSGISQC GYSSTIVHVP PPETARDATL FCEPWTLNMQ
661PAPLTSEEFK HVGLTAAVLS AHTQKEEQNY VDKFREKILS SPYSSYLQQE SRSKAKYSYF
721QGDSTSKQTR SAGCRKGKHK RKKLPEPPDS SSSNTGSGPR RGAHQNAQPC CPSAASSPHT
781SSPTFPPAAM VPSQAPYLVP AFPLPAATSP GREYAAPGTA PEGLHGLPLS EGLQPYPAFP
841FPYLDTFMTV FLPDPPVCPL LSPSFLPCPF LGATASSAIS PSMSSAMSPT LDPPPSVTSQ
901RREEEKWEAQ SEGHPFITSR SSSPLQLNLL QEEMPRPSES PDQMRRNTCP QTEYCVTGNN
961GSESSPATTG ALSTGSPPRE NPSHPTASAL STGSPPMKNP SHPTASALST GSPPMKNPSH
1021PTASTLSMGL PPSRTPSHPT ATVLSTGSPP SESPSRTGSA ASGSSDSSIY LTSSVYSSKI
1081SQNGQQSQDV QKKETFPNVA EEPIWRMIRQ TPERILMTYQ VPERVKEVVL KEDLEKLESM
1141RQQQPQFSHG QKEELAKVYN WIQSQTVTQE IDIQACVTCE NEDSADGAAT SCGQVLVEDS
1201C
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1201 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.