Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PELI3 / Pellino 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106768-100
Catalog Number: LS-G106768
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PELI3 (NM_145065), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PELI3 | Pellino-3 | Pellino homolog 3 | Protein pellino homolog 3 | Pellino homolog 3 (Drosophila)
- Origin Species
- Human
- SwissProt
- Q8N2H9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVLEGNPEVG SPRTSDLQHR GNKGSCVLSS PGEDAQPGEE PIKYGELIVL GCCEEGGEET61EAQRGEVTGP RAHSCYNGCL ASGDKGRRRS RLALSRRSHA NGVKPDVMHH ISTPLVSKAL121SNRGQHSISY TLSRSHSVIV EYTHDSDTDM FQIGRSTENM IDFVVTDTSP GGGAAEGPSA181QSTISRYACR ILCDRRPPYT ARIYAAGFDA SSNIFLGERA AKWRTPDGLM DGLTTNGVLV241MHPAGGFSED SAPGVWREIS VCGNVYTLRD SRSAQQRGKL VENESNVLQD GSLIDLCGAT301LLWRTPAGLL RAPTLKQLEA QRQEANAARP QCPVGLSTLA FPSPARGRTA PDKQQPWVYV361RCGHVHGYHG WGCRRERGPQ ERECPLCRLV GPYVPLWLGQ EAGLCLDPGP PSHAFAPCGH421VCSEKTARYW AQTPLPHGTH AFHAACPFCG AWLTGEHGCV RLIFQGPLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
469 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.