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PDZRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDZRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDZRN3 antibodies →
$725.00each
Catalog Number: LS-G113529-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMCAP3(PDZRN3) (NM_015009), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ligand of Numb protein X 3 | PDZRN3 | KIAA1095 | Protein SEMACAP3 | SEMCAP3 | LNX3 | SEMACAP3
Origin Species
Human
SwissProt
Q9UPQ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGFELDRFDG DVDPDLKCAL CHKVLEDPLT TPCGHVFCAG CVLPWVVQEG SCPARCRGRL
61SAKELNHVLP LKRLILKLDI KCAYATRGCG RVVKLQQLPE HLERCDFAPA RCRHAGCGQV
121LLRRDVEAHM RDACDARPVG RCQEGCGLPL THGEQRAGGH CCARALRAHN GALQARLGAL
181HKALKKEALR AGKREKSLVA QLAAAQLELQ MTALRYQKKF TEYSARLDSL SRCVAAPPGG
241KGEETKSLTL VLHRDSGSLG FNIIGGRPSV DNHDGSSSEG IFVSKIVDSG PAAKEGGLQI
301HDRIIEVNGR DLSRATHDQA VEAFKTAKEP IVVQVLRRTP RTKMFTPPSE SQLVDTGTQT
361DITFEHIMAL TKMSSPSPPV LDPYLLPEEH PSAHEYYDPN DYIGDIHQEM DREELELEEV
421DLYRMNSQDK LGLTVCYRTD DEDDIGIYIS EIDPNSIAAK DGRIREGDRI IQINGIEVQN
481REEAVALLTS EENKNFSLLI ARPELQLDEG WMDDDRNDFL DDLHMDMLEE QHHQAMQFTA
541SVLQQKKHDE DGGTTDTATI LSNQHEKDSG VGRTDESTRN DESSEQENNG DDATASSNPL
601AGQRKLTCSQ DTLGSGDLPF SNESFISADC TDADYLGIPV DECERFRELL ELKCQVKSAT
661PYGLYYPSGP LDAGKSDPES VDKELELLNE ELRSIELECL SIVRAHKMQQ LKEQYRESWM
721LHNSGFRNYN TSIDVRRHEL SDITELPEKS DKDSSSAYNT GESCRSTPLT LEISPDNSLR
781RAAEGISCPS SEGAVGTTEA YGPASKNLLS ITEDPEVGTP TYSPSLKELD PNQPLESKER
841RASDGSRSPT PSQKLGSAYL PSYHHSPYKH AHIPAHAQHY QSYMQLIQQK SAVEYAQSQM
901SLVSMCKDLS SPTPSEPRME WKVKIRSDGT RYITKRPVRD RLLRERALKI REERSGMTTD
961DDAVSEMKMG RYWSKEERKQ HLVKAKEQRR RREFMMQSRL DCLKEQQAAD DRKEMNILEL
1021SHKKMMKKRN KKIFDNWMTI QELLTHGTKS PDGTRVYNSF LSVTTVTRR
LEQKLISEEDLAANDILDYKDDDDKV

1069 aa + 26 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.