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PDZD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDZD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108780-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDZD4 (NM_032512), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PDZD4 | PDZK4 | PDZ domain containing 4 | KIAA1444 | LU1 | PDZRN4L
Origin Species
Human
NCBI GenBank
NM_032512
SwissProt
Q76G19

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCNMCVVQK PEEQYKVMLQ VNGKELSKLS QEQTLQALRS SKEPLVIQVL RRSPRLRGDS
61SCHDLQLVDS GTQTDITFEH IMALGKLRPP TPPMVILEPP PISHEYYDPA EFMEGGPQEA
121DRLDELEYEE VELYKSSHRD KLGLMVCYRT DDEEDLGIYV GEVNPNSIAA KDGRIREGDR
181IIQINGVDVQ NREEAVAILS QEENTNISLL VARPESQLAK RWKDSDRDDF LDDFGSENEG
241ELRARKLKSP PAQQPGNEEE KGAPDAGPGL SNSQELDSGV GRTDESTRNE ESSEHDLLGD
301EPPSSTNTPG SLRKFGLQGD ALQSRDFHFS MDSLLAEGAG LGGGDVPGLT DEEYERYREL
361LEIKCHLENG NQLGLLFPRA SGGNSALDVN RNESLGHEMA MLEEELRHLE FKCRNILRAQ
421KMQQLRERCM KAWLLEEESL YDLAASEPKK HELSDISELP EKSDKDSTSA YNTGESCRST
481PLLVEPLPES PLRRAMAGNS NLNRTPPGPA VATPAKAAPP PGSPAKFRSL SRDPEAGRRQ
541HAEERGRRNP KTGLTLERVG PESSPYLSRR HRGQGQEGEH YHSCVQLAPT RGLEELGHGP
601LSLAGGPRVG GVAAAATEAP RMEWKVKVRS DGTRYVAKRP VRDRLLKARA LKIREERSGM
661TTDDDAVSEM KMGRYWSKEE RKQHLIRARE QRKRREFMMQ SRLECLREQQ NGDSKPELNI
721IALSHRKTMK KRNKKILDNW ITIQEMLAHG ARSADGKRVY NPLLSVTTV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

769 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.