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PDZD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDZD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129927-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDZD3 (NM_001168468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IKEPP | NHERF-4 | PDZ domain containing 2 | PDZD3 | PDZK2 | NaPi-Cap2 | NHERF4 | PDZ domain containing 3
Origin Species
Human
NCBI GenBank
NM_024791

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKAADLQDT ASLTLKFKFN PKLGIDNPVL SLAEDHDPYD PWSLERPRFC LLSKEEGKSF
61GFHLQQELGR AGHVVCRVDP GTSAQRQGLQ EGDRILAVNN DVVEHEDYAV VVRRIRASSP
121RVLLTVLARH AHDVARAQLG EDAHLCPTLG PGVRPRLCHI VKDEGGFGFS VTHGNQGPFW
181LVLSTGGAAE RAGVPPGARL LEVNGVSVEK FTHNQLTRKL WQSGQQVTLL VAGPEVEEQC
241RQLGLPLAAP LAEGWALPTK PRCLHLEKGP QGFGFLLREE KGLDGRPGQF LWEVDPGLPA
301KKAGMQAGDR LVAVAGESVE GLGHEETVSR IQGQGSCVSL TVVDPEADRF FSMVRLSPLL
361FLENTEAPAS PRGSSSASLV ETEDPSLEDT SVPSVPLGSR QCFLYPGPGG SYGFRLSCVA
421SGPRLFISQV TPGGSAARAG LQVGDVILEV NGYPVGGQND LERLQQLPEA EPPLCLKLAA
481RSLRGLEAWI PPGAAEDWAL ASDLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

505 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.