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PDPR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDPR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDPR antibodies →
$725.00each
Catalog Number: LS-G112075-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDPR (NM_017990), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1990 | PDP3 | PDPR
Origin Species
Human
NCBI GenBank
NM_017990
SwissProt
Q8NCN5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMFYRLLSIV GRQRASPGWQ NWSSARNSTS AAEARSMALP TQAQVVICGG GITGTSVAYH
61LSKMGWKDIV LLEQGRLAAG STRFCAGILS TARHLTIEQK MADYSNKLYY QLEQETGIQT
121GYTRTGSIFL AQTQDRLISL KRINAGLNVI GIPSEIISPK KVAELHHLLN VHDLVGAMHV
181PEDAVVSSAD VALALASAAS QNGVQIYDRT SVLHVMVKKG QVTGVETDKG QIECQYFVNC
241AGQWAYELGL SNEEPVSIPL HACEHFYLLT RPLETPLQSS TPTIVDADGR IYIRNWQGGI
301LSGGFEKNPK PIFTEGKNQL EIQNLQEDWD HFEPLLSSLL RRMPELETLE IMKLVNCPET
361FTPDMRCIMG ESPAVQGYFV LAGMNSAGLS FGGGAGKYLA EWMVHGYPSE NVWELDLKRF
421GALQSSRTFL RHRVMEVMPL MYDLKVPRWD FQTGRQLRTS PLYDRLDAQG ARWMEKHGFE
481RPKYFVPPDK DLLALEQSKT FYKPDWFDIV ESEVKCCKEA VCVIDMSSFT KFEITSTGDQ
541ALEVLQYLFS NDLDVPVGHI VHTGMLNEGG GYENDCSIAR LNKRSFFMIS PTDQQVHCWA
601WLKKHMPKDS NLLLEDVTWK YTALNLIGPR AVDVLSELSY APMTPDHFPS LFCKEMSVGY
661ANGIRVMSMT HTGEPGFMLY IPIEYALHVY NEVMSVGQKY GIRNAGYYAL RSLRIEKFFA
721FWGQDINNLT TPLECGRESR VKLEKGMDFI GRDALLQQKQ NGVYKRLTMF ILDDHDSDLD
781LWPWWGEPIY RNGQYVGKTT SSAYSYSLER HVCLGFVHNF SEDTGEEQVV TADFINRGEY
841EIDIAGYRFQ AKAKLYPVAS LFTQKRRKDD MELSDLHGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

879 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.