Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PDLIM7 / Enigma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103079-100
Catalog Number: LS-G103079
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDLIM7 (NM_203352), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ENIGMA | LMP1 | Lim mineralization protein 3 | PDZ and LIM domain 7 (enigma) | PDLIM7 | Protein enigma | LIM domain protein | LMP | PDZ and LIM domain protein 7 | LIM mineralization protein | LMP3
- Origin Species
- Human
- NCBI GenBank
- NM_005451
- SwissProt
- Q9NR12
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSFKVVLEG PAPWGFRLQG GKDFNVPLSI SRLTPGGKAA QAGVAVGDWV LSIDGENAGS61LTHIEAQNKI RACGERLSLG LSRAQPVQSK PQKVQTPDKQ PLRPLVPDAS KQRLMENTED121WRPRPGTGQS RSFRILAHLT GTEFMQDPDE EHLKKSSQVP RTEAPAPASS TPQEPWPGPT181APSPTSRPPW AVDPAFAERY APDKTSTVLT RHSQPATPTP LQSRTSIVQA AAGGVPGGGS241NNGKTPVCHQ CHKVIRGRYL VALGHAYHPE EFVCSQCGKV LEEGGFFEEK GAIFCPPCYD301VRYAPSCAKC KKKITGEIMH ALKMTWHVHC FTCAACKTPI RNRAFYMEEG VPYCERDYEK361MFGTKCHGCD FKIDAGDRFL EALGFSWHDT CFVCAICQIN LEGKTFYSKK DRPLCKSHAF421SHVSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
423 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.