Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PDLIM2 / SLIM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127528-100
Catalog Number: LS-G127528
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDLIM2 (NM_176871), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MYSTIQUE | PDLIM2 | PDZ-LIM protein mystique | PDZ and LIM domain protein 2 | SLIM
- Origin Species
- Human
- NCBI GenBank
- NM_021630
- SwissProt
- Q96JY6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALTVDVAGP APWGFRITGG RDFHTPIMVT KVAERGKAKD ADLRPGDIIV AINGESAEGM61LHAEAQSKIR QSPSPLRLQL DRSQATSPGQ TNGDSSLEVL ATRFQGSVRT YTESQSSLRS121SYSSPTSLSP RAGSPFSPPP SSSSLTGEAA ISRSFQSLAC SPGLPAADRL SYSGRPGSRQ181AGLGRAGDSA VLVLPPSPGP RSSRPSMDSE GGSLLLDEDS EVFKMLQENR EGRAAPRQSS241SFRLLQEALE AEERGGTPAF LPSSLSPQSS LPASRALATP PKLHTCEKCS TSIANQAVRI301QEGRYRHPGC YTCADCGLNL KMRGHFWEDA CAMEGMRLSL EALEGMVEGA KRRDRRKTRR361PIQPSWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
366 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.