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PDILT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDILT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDILT antibodies →
$504.00each
Catalog Number: LS-G105149-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDILT (NM_174924), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PDILT | PDIA7
Origin Species
Human
SwissProt
Q8N807

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLLWMPLLL VAACVSAVHS SPEVNAGVSS IHITKPVHIL EERSLLVLTP AGLTQMLNQT
61RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK VDITIEKELQ QEFGITKAPE
121LKLFFEGNRS EPISCKGVVE SAALVVWLRR QISQKAFLFN SSEQVAEFVI SRPLVIVGFF
181QDLEEEVAEL FYDVIKDFPE LTFGVITIGN VIGRFHVTLD SVLVFKKGKI VNRQKLINDS
241TNKQELNRVI KQHLTDFVIE YNTENKDLIS ELHIMSHMLL FVSKSSESYG IIIQHYKLAS
301KEFQNKILFI LVDADEPRNG RVFKYFRVTE VDIPSVQILN LSSDARYKMP SDDITYESLK
361KFGRSFLSKN ATKHQSSEEI PKYWDQGLVK QLVGKNFNVV VFDKEKDVFV MFYAPWSKKC
421KMLFPLLEEL GRKYQNHSTI IIAKIDVTAN DIQLMYLDRY PFFRLFPSGS QQAVLYKGEH
481TLKGFSDFLE SHIKTKIEDE DELLSVEQNE VIEEEVLAEE KEVPMMRKGL PEQQSPELEN
541MTKYVSKLEE PAGKKKTSEE VVVVVAKPKG PPVQKKKPKV KEEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.