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PDE4D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE4D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G100752-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE4D (NM_006203), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACRDYS2 | DPDE3 | HSPDE4D | PDE4D | PDE43 | Pde-ivd | PDE4DN2 | Phosphodiesterase 4d | STRK1
Origin Species
Human
NCBI GenBank
NM_006203
SwissProt
Q08499

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHVNNFPFRR HSWICFDVDN GTSAGRSPLD PMTSPGSGLI LQANFVHSQR RESFLYRSDS
61DYDLSPKSMS RNSSIASDIH GDDLIVTPFA QVLASLRTVR NNFAALTNLQ DRAPSKRSPM
121CNQPSINKAT ITEEAYQKLA SETLEELDWC LDQLETLQTR HSVSEMASNK FKRMLNRELT
181HLSEMSRSGN QVSEFISNTF LDKQHEVEIP SPTQKEKEKK KRPMSQISGV KKLMHSSSLT
241NSSIPRFGVK TEQEDVLAKE LEDVNKWGLH VFRIAELSGN RPLTVIMHTI FQERDLLKTF
301KIPVDTLITY LMTLEDHYHA DVAYHNNIHA ADVVQSTHVL LSTPALEAVF TDLEILAAIF
361ASAIHDVDHP GVSNQFLINT NSELALMYND SSVLENHHLA VGFKLLQEEN CDIFQNLTKK
421QRQSLRKMVI DIVLATDMSK HMNLLADLKT MVETKKVTSS GVLLLDNYSD RIQVLQNMVH
481CADLSNPTKP LQLYRQWTDR IMEEFFRQGD RERERGMEIS PMCDKHNASV EKSQVGFIDY
541IVHPLWETWA DLVHPDAQDI LDTLEDNREW YQSTIPQSPS PAPDDPEEGR QGQTEKFQFE
601LTLEEDGESD TEKDSGSQVE EDTSCSDSKT LCTQDSESTE IPLDEQVEEE AVGEEEESQP
661EACVIDDRSP DT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

672 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.