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PDE4C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE4C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDE4C antibodies →
$725.00each
Catalog Number: LS-G118992-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE4C (NM_001098818), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DPDE1 | Pde-ivc | PDE4C | PDE21 | Phosphodiesterase 4c | Pde4c2
Origin Species
Human
NCBI GenBank
NM_000923
SwissProt
Q08493

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQGPPAPAPV PGPGSPRGSP RGSPGLFRKL LVNQSIRLQR RFTVAHPLCF DLENGLSCGR
61RALDPQSSPG LGRIMQAPVP HSQRRESFLY RSDSDYELSP KAMSRNSSVA SDLHGEDMIV
121TPFAQVLASL RTVRSNVAAL ARQQCLGAAK QGPVGNPSSS NQLPPAEDTG QKLALETLDE
181LDWCLDQLET LQTRHSVGEM ASNKFKRILN RELTHLSETS RSGNQVSEYI SRTFLDQQTE
241VELPKVTAEE APQPMSRISG LHGLCHSASL SSATVPRFGV QTDQEEQLAK ELEDTNKWGL
301DVFKVAELSG NRPLTAIIFS IFQERDLLKT FQIPADTLAT YLLMLEGHYH ANVAYHNSLH
361AADVAQSTHV LLATPALEAV FTDLEILAAL FASAIHDVDH PGVSNQFLIN TNSELALMYN
421DASVLENHHL AVGFKLLQAE NCDIFQNLSA KQRLSLRRMV IDMVLATDMS KHMNLLADLK
481TMVETKKVTS LGVLLLDNYS DRIQVLQNLV HCADLSNPTK PLPLYRQWTD RIMAEFFQQG
541DRERESGLDI SPMCDKHTAS VEKSQVGFID YIAHPLWETW ADLVHPDAQD LLDTLEDNRE
601WYQSKIPRSP SDLTNPERDG PDRFQFELTL EEAEEEDEEE EEEGEETALA KEALELPDTE
661LLSPEAGPDP GDLPLDNQRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.