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PDE1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128199-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE1B (NM_001165975), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
63 kDa Cam-PDE | Cam-PDE 1B | PDES1B | PDE1B1 | PDE1B | PDE1B2
Origin Species
Human
NCBI GenBank
NM_000924
SwissProt
Q01064

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MANPVPVQRS HLQGPILRLR YMVKQLENGE INIEELKKNL EYTASLLEAV YIDETRQILD
61TEDELQELRS DAVPSEVRDW LASTFTQQAR AKGRRAEEKP KFRSIVHAVQ AGIFVERMFR
121RTYTSVGPTY STAVLNCLKN LDLWCFDVFS LNQAADDHAL RTIVFELLTR HNLISRFKIP
181TVFLMSFLDA LETGYGKYKN PYHNQIHAAD VTQTVHCFLL RTGMVHCLSE IELLAIIFAA
241AIHDYEHTGT TNSFHIQTKS ECAIVYNDRS VLENHHISSV FRLMQDDEMN IFINLTKDEF
301VELRALVIEM VLATDMSCHF QQVKTMKTAL QQLERIDKPK ALSLLLHAAD ISHPTKQWLV
361HSRWTKALME EFFRQGDKEA ELGLPFSPLC DRTSTLVAQS QIGFIDFIVE PTFSVLTDVA
421EKSVQPLADE DSKSKNQPSF QWRQPSLDVE VGDPNPDVVS FRSTWVKRIQ ENKQKWKERA
481ASGITNQMSI DELSPCEEEA PPSPAEDEHN QNGNLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.