LSBio
PDE1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDE1A antibodies →
$504.00each
Catalog Number: LS-G100439-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE1A (NM_005019), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
61 kDa Cam-PDE | 59 kDa CaM-PDE | Cam-PDE 1A | HCAM-1 | HCAM1 | HSPDE1A | Phosphodiesterase 1a | PDE1A | CAM-PDE-1A
Origin Species
Human
NCBI GenBank
NM_005019
SwissProt
P54750

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSSATEIEE LENTTFKYLT GEQTEKMWQR LKGILRCLVK QLERGDVNVV DLKKNIEYAA
61SVLEAVYIDE TRRLLDTEDE LSDIQTDSVP SEVRDWLAST FTRKMGMTKK KPEEKPKFRS
121IVHAVQAGIF VERMYRKTYH MVGLAYPAAV IVTLKDVDKW SFDVFALNEA SGEHSLKFMI
181YELFTRYDLI NRFKIPVSCL ITFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI
241MHWLTELEIL AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
301QEEEMNILIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE GIDRAKTMSL
361ILHAADISHP AKSWKLHYRW TMALMEEFFL QGDKEAELGL PFSPLCDRKS TMVAQSQIGF
421IDFIVEPTFS LLTDSTEKIV IPLIEEASKA ETSSYVASSS TTIVGLHIAD ALRRSNTKGS
481MSDGSYSPDY SLAAVDLKSF KNNLVDIIQQ NKERWKELAA QGESDLHKNS EDLVNAEEKH
541DETHS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.