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PDE12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDE12 antibodies →
$725.00each
Catalog Number: LS-G104806-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE12 (NM_177966), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
2'-PDE | 2 | 5-phosphodiesterase 12 | 2-PDE | 2-phosphodiesterase | PDE12 | Phosphodiesterase 12
Origin Species
Human
NCBI GenBank
AK001526
SwissProt
Q6L8Q7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWRLPGARAA LRVIRTAVEK LSRAEAGSQT AAGAMERAVV RCVPSEPKLS LSFALADGSH
61KNMQRDQSEP LGRVLSRIAT NALKGHAKAA AAKKSRKSRP NASGGAACSG PGPEPAVFCE
121PVVKLYYREE AVAEDVLNVD AWQDGAVLQI GDVKYKVERN PPAFTELQLP RYIMAGFPVC
181PKLSLEFGDP ASSLFRWYKE AKPGAAEPEV GVPSSLSPSS PSSSWTETDV EERVYTPSNA
241DIGLRLKLHC TPGDGQRFGH SRELESVCVV EAGPGTCTFD HRHLYTKKVT EDALIRTVSY
301NILADTYAQT EFSRTVLYPY CAPYALELDY RQNLIQKELT GYNADVICLQ EVDRAVFSDS
361LVPALEAFGL EGVFRIKQHE GLATFYRKSK FSLLSQHDIS FYEALESDPL HKELLEKLVL
421YPSAQEKVLQ RSSVLQVSVL QSTKDSSKRI CVANTHLYWH PKGGYIRLIQ MAVALAHIRH
481VSCDLYPGIP VIFCGDFNST PSTGMYHFVI NGSIPEDHED WASNGEEERC NMSLTHFFKL
541KSACGEPAYT NYVGGFHGCL DYIFIDLNAL EVEQVIPLPS HEEVTTHQAL PSVSHPSDHI
601ALVCDLKWK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

609 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.