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PDCD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDCD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDCD7 (NM_005707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Apoptosis-related protein ES18 | HES18 | PDCD7 | Programmed cell death 7 | U11/U12 snRNP 59K | ES18
Origin Species
Human
NCBI GenBank
NM_005707
SwissProt
Q8N8D1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALPPFFGQG RPGPPPPQPP PPAPFGCPPP PLPSPAFPPP LPQRPGPFPG ASAPFLQPPL
61ALQPRASAEA SRGGGGAGAF YPVPPPPLPP PPPQCRPFPG TDAGERPRPP PPGPGPPWSP
121RWPEAPPPPA DVLGDAALQR LRDRQWLEAV FGTPRRAGCP VPQRTHAGPS LGEVRARLLR
181ALRLVRRLRG LSQALREAEA DGAAWVLLYS QTAPLRAELA ERLQPLTQAA YVGEARRRLE
241RVRRRRLRLR ERAREREAER EAEAARAVER EQEIDRWRVK CVQEVEEKKR EQELKAAADG
301VLSEVRKKQA DTKRMVDILR ALEKLRKLRK EAAARKGVCP PASADETFTH HLQRLRKLIK
361KRSELYEAEE RALRVMLEGE QEEERKRELE KKQRKEKEKI LLQKREIESK LFGDPDEFPL
421AHLLEPFRQY YLQAEHSLPA LIQIRHDWDQ YLVPSDHPKG NFVPQGWVLP PLPSNDIWAT
481AVKLH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.