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PCYOX1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCYOX1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCYOX1L antibodies →
$725.00each
Catalog Number: LS-G110094-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCYOX1L (NM_024028), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCYOX1L | Prenylcysteine oxidase-like | Prenylcysteine oxidase 1 like
Origin Species
Human
NCBI GenBank
NM_024028
SwissProt
Q8NBM8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARAPPLLAA LTALLAAAAA GGDAPPGKIA VVGAGIGGSA VAHFLQQHFG PRVQIDVYEK
61GTVGGRLATI SVNKQHYESG AASFHSLSLH MQDFVKLLGL RHRREVVGRS AIFGGEHFML
121EETDWYLLNL FRLWWHYGIS FLRLQMWVEE VMEKFMRIYK YQAHGYAFSG VEELLYSLGE
181STFVNMTQHS VAESLLQVGV TQRFIDDVVS AVLRASYGQS AAMPAFAGAM SLAGAQGSLW
241SVEGGNKLVC SGLLKLTKAN VIHATVTSVT LHSTEGKALY QVAYENEVGN SSDFYDIVVI
301ATPLHLDNSS SNLTFAGFHP PIDDVQGSFQ PTVVSLVHGY LNSSYFGFPD PKLFPFANIL
361TTDFPSFFCT LDNICPVNIS ASFRRKQPQE AAVWRVQSPK PLFRTQLKTL FRSYYSVQTA
421EWQAHPLYGS RPTLPRFALH DQLFYLNALE WAASSVEVMA VAAKNVALLA YNRWYQDLDK
481IDQKDLMHKV KTEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

494 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.