Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PCTK3 / CDK18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117867-100
Catalog Number: LS-G117867
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCTAIRE3(CDK18) (NM_002596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Pctaire-3 | PCTK3 | PCTAIRE-motif protein kinase 3 | PCTAIRE3 | CDK18 | Cyclin-dependent kinase 18 | PCTAIRE | PCTAIRE protein kinase 3
- Origin Species
- Human
- NCBI GenBank
- BC011526
- SwissProt
- Q07002
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNKMKNFKRR FSLSVPRTET IEESLAEFTE QFNQLHNRRN ENLQLGPLGR DPPQECSTFS61PTDSGEEPGQ LSPGVQFQRR QNQRRFSMED VSKRLSLPMD IRLPQEFLQK LQMESPDLPK121PLSRMSRRAS LSDIGFGKLE TYVKLDKLGE GTYATVFKGR SKLMENLVAL KEIRLEHEEG181APCTAIREVS LLKNLKHANI VTLHDLIHTD RSLTLVFEYL DSDLKQYLDH CGNLMSMHNV241KIFMFQLLRG LAYCHHRKIL HRDLKPQNLL INERGELKLA DFGLARAKSV PTKTYSNEVV301TLWYRPPDVL LGSTEYSTPI DMWGVGCIHY EMATGRPLFP GSTVKEELHL IFRLLGTPTE361ETWPGVTAFS EFRTYSFPCY LPQPLINHAP RLDTDGIHLL SSLLLYESKS RMSAEAALSH421SYFRSLGERV HQLEDTASIF SLKEIQLQKD PGYRGLAFQQ PGRGKNRRQS IFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
472 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.