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PCIF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCIF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCIF1 antibodies →
$504.00each
Catalog Number: LS-G110465-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCIF1 (NM_022104), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf67 | BA465L10.1 | PCIF1
Origin Species
Human
SwissProt
Q9H4Z3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MANENHGSPR EEASLLSHSP GTSNQSQPCS PKPIRLVQDL PEELVHAGWE KCWSRRENRP
61YYFNRFTNQS LWEMPVLGQH DVISDPLGLN ATPLPQDSSL VETPPAENKP RKRQLSEEQP
121SGNGVKKPKI EIPVTPTGQS VPSSPSIPGT PTLKMWGTSP EDKQQAALLR PTEVYWDLDI
181QTNAVIKHRG PSEVLPPHPE VELLRSQLIL KLRQHYRELC QQREGIEPPR ESFNRWMLER
241KVVDKGSDPL LPSNCEPVVS PSMFREIMND IPIRLSRIKF REEAKRLLFK YAEAARRLIE
301SRSASPDSRK VVKWNVEDTF SWLRKDHSAS KEDYMDRLEH LRRQCGPHVS AAAKDSVEGI
361CSKIYHISLE YVKRIREKHL AILKENNISE EVEAPEVEPR LVYCYPVRLA VSAPPMPSVE
421MHMENNVVCI RYKGEMVKVS RNYFSKLWLL YRYSCIDDSA FERFLPRVWC LLRRYQMMFG
481VGLYEGTGLQ GSLPVHVFEA LHRLFGVSFE CFASPLNCYF RQYCSAFPDT DGYFGSRGPC
541LDFAPLSGSF EANPPFCEEL MDAMVSHFER LLESSPEPLS FIVFIPEWRE PPTPALTRME
601QSRFKRHQLI LPAFEHEYRS GSQHICKKEE MHYKAVHNTA VLFLQNDPGF AKWAPTPERL
661QELSAAYRQS GRSHSSGSSS SSSSEAKDRD SGREQGPSRE PHPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

704 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.