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PCDHGC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGC4 antibodies →
$725.00each
Catalog Number: LS-G108842-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGC4 (NM_032406), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-GAMMA-C4 | PCDHGC4 | Protocadherin gamma-C4
Origin Species
Human
NCBI GenBank
NM_018928
SwissProt
Q9Y5F7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRKVRSWTE IWRWATLLFL FYHLGYVCGQ IRYPVPEESQ EGTFVGNVAQ DFLLDTDSLS
61ARRLQVAGEV NQRHFRVDLD SGALLIKNPI DREALCGLSA SCIVPLEFVT EGPLEMYRAE
121VEIVDVNDHA PRFPRQQLDL EIGEAAPPGQ RFPLEKAQDA DVGSNSISSY RLSSNEHFAL
181DVKKRSDGSL VPELLLEKPL DREKQSDYRL VLTAVDGGNP PRSGTAELRV SVLDVNDNAP
241AFQQSSYRIS VLESAPAGMV LIQLNASDPD LGPSGNVTFY FSGHTPDRVR NLFSLHPTTG
301KLTLLGPLDF ESENYYEFDV RARDGGSPAM EQHCSLRVDL LDVNDNAPYI TVTSELGTLP
361ESAEPGTVVA LISVQDPDSG SNGDVSLRIP DHLPFALKSA FRNQFSLVTA GPLDREAKSS
421YDIMVTASDA GNPPLSTHRT IFLNISDVND NPPSFFQRSH EVFVPENNRP GDLLCSLAAS
481DPDSGLNALI SYSLLEPRNR DVSASSFISL NPQTGAVHAT RSFDYEQTQT LQFEVQARDR
541GNPPLSSTVT VRLFVLDLND NAPAVLRPRA RPGSLCPQAL PPSVGAGHLI TKVTAVDLDS
601GYNAWVSYQL LEAPDPSLFA VSRYAGEVRT AVPIPADLPP QKLVIVVKDS GSPPLSTSVT
661LLVSLEEDTH PVVPDLRESS APREGESRLT LYLAVSLVAI CFVSFGSFVA LLSKCLRGAA
721CGVTCFPAGT CACLTRSRRR EGLPPSNGIL RIQLGSDDPI KFVDVGGHSH GCTPLASAPT
781RSDSFMMVKS PSAPMAGEPV RPSCPPSDLL YGLEVRPLQA QQMLEGYSDP GIWLGHVLES
841TGLSVSAHSD VTIFVRGNYV VDAVLCNCFV N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

871 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.