Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PCDHGC3 / PCDH2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117883-100
Catalog Number: LS-G117883
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGC3 (NM_002588), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin-like 2 | PCDH-GAMMA-C3 | PCDHGC3 | PC-43 | PCDH2 | Protocadherin 43 | Protocadherin gamma-C3 | Protocadherin-2 | Protocadherin 2 | PC43 | Protocadherin-43
- Origin Species
- Human
- NCBI GenBank
- NM_002588
- SwissProt
- Q9UN70
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.08 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVPEAWRSGL VSTGRVVGVL LLLGALNKAS TVIHYEIPEE REKGFAVGNV VANLGLDLGS61LSARRFRVVS GASRRFFEVN RETGEMFVND RLDREELCGT LPSCTVTLEL VVENPLELFS121VEVVIQDIND NNPAFPTQEM KLEISEAVAP GTRFPLESAH DPDVGSNSLQ TYELSRNEYF181ALRVQTREDS TKYAELVLER ALDREREPSL QLVLTALDGG TPALSASLPI HIKVLDANDN241APVFNQSLYR ARVLEDAPSG TRVVQVLATD LDEGPNGEII YSFGSHNRAG VRQLFALDLV301TGMLTIKGRL DFEDTKLHEI YIQAKDKGAN PEGAHCKVLV EVVDVNDNAP EITVTSVYSP361VPEDAPLGTV IALLSVTDLD AGENGLVTCE VPPGLPFSLT SSLKNYFTLK TSADLDRETV421PEYNLSITAR DAGTPSLSAL TIVRVQVSDI NDNPPQSSQS SYDVYIEENN LPGAPILNLS481VWDPDAPQNA RLSFFLLEQG AETGLVGRYF TINRDNGIVS SLVPLDYEDR REFELTAHIS541DGGTPVLATN ISVNIFVTDR NDNAPQVLYP RPGGSSVEML PRGTSAGHLV SRVVGWDADA601GHNAWLSYSL LGSPNQSLFA IGLHTGQIST ARPVQDTDSP RQTLTVLIKD NGEPSLSTTA661TLTVSVTEDS PEARAEFPSG SAPREQKKNL TFYLLLSLIL VSVGFVVTVF GVIIFKVYKW721KQSRDLYRAP VSSLYRTPGP SLHADAVRGG LMSPHLYHQV YLTTDSRRSD PLLKKPGAAS781PLASRQNTLR SCDPVFYRQV LGAESAPPGQ QAPPNTDWRF SQAQRPGTSG SQNGDDTGTW841PNNQFDTEML QAMILASASE AADGSSTLGG GAGTMGLSAR YGPQFTLQHV PDYRQNVYIP901GSNATLTNAA GKRDGKAPAG GNGNKKKSGK KEKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
934 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.