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PCDHGB7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGB7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGB7 antibodies →
$725.00each
Catalog Number: LS-G109078-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGB7 (NM_032101), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cadherin ME6 | ME6 | PCDHGB7 | PCDH-GAMMA-B7 | Protocadherin gamma-B7
Origin Species
Human
NCBI GenBank
NM_018927
SwissProt
Q9Y5F8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGGSCAQRRR AGPRQVLFPL LLPLFYPTLC EPIRYSIPEE LAKGSVVGNL AKDLGLSVLD
61VSARELRVSA EKLHFSVDAQ SGDLLVKDRI DREQICKERR RCELQLEAVV ENPLNIFHVI
121VVIEDVNDHA PQFRKDEINL EISESVSLGM GTILESAEDP DISMNSLSKY QLSPNEYFSL
181VEKDNPDGGK YPELVLQKTL DRETQSAHHL VLTALDGGDP PRSGTAQIRI LVIDANDNPP
241VFSQDVYRVS LREDVPPGTS ILRVKATDQD EGINSEITYS FFGVADKAQH VFSLDYTTGN
301ILTQQPLDFE EVERYTINIE AKDRGSLSTR CKVIVEVVDE NDNSPEIIIT SLSDQIMEDS
361PPGVVVALFK TRDQDSGENG EVRCSLSRGV PFKIHSSSNN YYKLVTDEAL DREQTPEYNV
421TIAATDRGKP PLSSSKTITL HITDVNDNAP VFGQSAYLVH VPENNQPGAS IAQVSASDPD
481FGLNGRVSYS LIASDLESRT LSSYVSVSAQ SGVVFAQRAF DHEQLRTFEL TLQARDQGSP
541ALSANVSLRV LVGDRNDNAP RVLYPALGPD GSALFDTVPR AAQPGYLVTK VVAVDADSGH
601NAWLSYHVVQ ASEPGLFSLG LRTGEVRMVR ALGDKDSVRQ RLLVAVRDGG QPPLSATATL
661HLVFADSLQE VLPDFSDHPT PSDSQAEMQF YLVVALALIS VLFLLAVILA IALRLRQSFS
721PTAGDCFESV LCSKSGPVGP PNYSEGTLPY AYNFCVPGDQ MNPEFNFFTS VDHCPATQDN
781LNKDSMLLAS ILTPSVEADK KILKQVSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

808 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.