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PCDHGB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117118-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGB4 (NM_003736), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cadherin 20 | Fibroblast cadherin-2 | PCDH-GAMMA-B4 | PCDHGB4 | Protocadherin gamma-B4 | Cadherin-20 | Fib2 | Fibroblast cadherin FIB2
Origin Species
Human
NCBI GenBank
NM_003736
SwissProt
Q9UN71

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.93 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSGAGELGR AERLPVLFLF LLSLFCPALC EQIRYRIPEE MPKGSVVGNL ATDLGFSVQE
61LPTRKLRVSS EKPYFTVSAE SGELLVSSRL DREEICGKKP ACALEFEAVA ENPLNFYHVN
121VEIEDINDHT PKFTQNSFEL QISESAQPGT RFILGSAHDA DIGSNTLQNY QLSPSDHFSL
181INKEKSDGSK YPEMVLKTPL DREKQKSYHL TLTALDFGAP PLSSTAQIHV LVTDANDNAP
241VFSQDVYRVS LSENVYPGTT VLQVTATDQD EGVNAEITFS FSEASQITQF DLNSNTGEIT
301VLNTLDFEEV KEYSIVLEAR DGGGMIAQCT VEVEVIDEND NAPEVIFQSL PNLIMEDAEL
361GTHIALLKVR DKDSRHNGEV TCKLEGDVPF KILTSSRNTY KLVTDAVLDR EQNPEYNITV
421TATDRGKPPL SSSSSITLHI GDVNDNAPVF SQSSYIVHVA ENNPPGASIS QVRASDPDLG
481PNGQVSYCIM ASDLEQRELS SYVSISAESG VVFAQRAFDH EQLRAFELTL QARDQGSPAL
541SANVSLRVLV DDRNDNAPRV LYPALGPDGS ALFDMVPHAA EPGYLVTKVV AVDADSGHNA
601WLSYHVLQAS EPGLFSLGLR TGEVRTARAL GDRDAVRQRL LVAVRDGGQP PLSATATLHL
661VFADSLQEVL PDITDRPDPS DLQAELQFYL VVALALISVL FLVAMILAIA LRLRRSSSPA
721SWSCFQPGLC VKSESVVPPN YSEGTLPYSY NLCVAHTGKT EFNFLKCSEQ LSSGQDILCG
781DSSGALFPLC NSSELTSHQQ APPNTDWRFS QAQRPGTSGS QNGDDTGTWP NNQFDTEMLQ
841AMILASASEA ADGSSTLGGG AGTMGLSARY GPQFTLQHVP DYRQNVYIPG SNATLTNAAG
901KRDGKAPAGG NGNKKKSGKK EKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

923 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.