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PCDHGB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109071-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGB2 (NM_032096), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-GAMMA-B2 | PCDHGB2 | Protocadherin gamma-B2
Origin Species
Human
NCBI GenBank
NM_018923
SwissProt
Q9Y5G2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKASSGRCGL VRWLQVLLPF LLSLFPGALP VQIRYSIPEE LAKNSVVGNL AKDLGLSVRD
61LPARKLRVSA EKEYFTVNPE SGDLLVSDRI DREQICGKQP LCVLDFDTVA ENPLNIFYIA
121VIVQDINDNT PLFKQTKINL KIGESTKPGT TFPLDPALDS DVGPNSLQRY HLNDNEYFDL
181AEKQTPDGRK YPELILKHSL DREEHSLHQL VLTAVDGGDP PQSGTTQIRI KVTDANDNPP
241VFSQDVYRVT LREDVPPGFF VLQVTATDRD EGINAEITYS FHNVDEQVKH FFNLNEKTGE
301ITTKDDLDFE IASSYTLSIE AKDPGDLAAH CSIQVEILDD NDCAPEVIVT SVSTPLPEDS
361PPGTVIALIK TRDRDSGENG EVYCQVLGNA KFILKSSSKN YYKLVTDGAL DREEIPEYNL
421TITATDGGKP PLSSSIIVTL HISDVNDNAP VFQQTSYMVH VAENNPPGAS IAQISASDPD
481LGPSGQVSYS IVASDLKPRE ILSYVSVSAQ SGVVFAQRAF DHEQLRAFEL TLQARDQGSP
541ALSANVSLRV LVGDLNDNAP RVLYPALGPD GSALFDMVPR AAEPGYLVTK VVAVDADSGH
601NAWLSYHVLQ ASEPGLFSLG LRTGEVRTAR ALGDRDAARQ RLLVAVRDGG QPPLSATATL
661HLIFADSLQE VLPDLSDRRE PSDPQAKLQF YLVVALALIS VLFFLAVILA ISLRLRLSSR
721SDAWDCFQPG LSSKPGPGVL PNYSEGTLPY SYNLCVASQS AKTEFNFLNI TPELVPAQDL
781VCDNASWEQN TNHGAAGVPF ASDTILKVSF N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

811 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.