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PCDHGA9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGA9 antibodies →
$725.00each
Catalog Number: LS-G111613-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA9 (NM_018921), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-GAMMA-A9 | Protocadherin gamma-A9 | PCDHGA9
Origin Species
Human
NCBI GenBank
AF152329
SwissProt
Q9Y5G4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPTKCQLR GRLVLLCSLL GMLWEARASQ IRYSVPEETE KGYIVGNISK DLALEPRELA
61ERRVRIVSRG RTQLFSLNPR SGTLVTAGRI DREELCAQSP RCLVNFKVLV EDRVKLYGIE
121IEVTDINDSA PKFQAESLEV KINEIAVPGA RYPLPEAIDP DVGVNSLQSY QLSPNHHFSL
181NVQTGDNGAI NPELVLERAL DREEATAHHL VLTASDGGEP RRSSTVRIHV TVLDTNDNAP
241VFAQRIYRVK VLENVPPGTW LLTATASDLD EGINGKVAYK FWKINEKQSL LFQLNENTGE
301ISTAKSLDYE ECSFYEMEIQ AEDGGGLKGW TKVLISVEDV NDNRPEVTIT SLFSPVREDA
361PQGTVILLFN AHDRDSGKNG QVVCSIQENL SFTLENSEED YYRLLTAQIL DREKASEYNI
421TVTATDRGTP PLSTEIHITL QVTDINDNPP AFSQASYSVY LPENNARGTS IFSVIAYDPD
481SNENSRVIYS LAEDTIQGSP LSTYVSINSD TGVLYALCSF DYEQFRDLQM QVTASDSGSP
541PLSSNVSLRL FVLDQNDNAP EILYPALPTD GSTGVELAPR SAEPGYLVTK VVAVDRDSGQ
601NAWLSYRLFK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAVQDHG QPPLSATVTL
661TVAIADSIPD ILADLGSLQI PADLEASDLT LYLVVAVAVV SCVFLTFVIT LLALRLRHWH
721SSHLLRATSD GLAGVPTSHF VGVDGVRAFL QTYSQEFSLT ADSRKSHLIF PQPNYADTLI
781SQQSCEKNEP LCVSVDSKFP IEDTPLVPQA PPNTDWRFSQ AQRPGTSGSQ NGDDTGTWPN
841NQFDTEMLQA MILASASEAA DGSSTLGGGA GTMGLSARYG PQFTLQHVPD YRQNVYIPGS
901NATLTNAAGK RDGKAPAGGN GNKKKSGKKE KK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.