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PCDHGA8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGA8 antibodies →
$725.00each
Catalog Number: LS-G109065-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA8 (NM_032088), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-GAMMA-A8 | KIAA0327 | PCDHGA8 | Protocadherin gamma-A8
Origin Species
Human
NCBI GenBank
NM_014004
SwissProt
Q9Y5G5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPQSRPRR GELILLCALL GTLWEIGRGQ IRYSVPEETD KGSFVGNISK DLGLDPRKLA
61KHGVRIVSRG RTQLFALNPR SGSLITAGRI DREELCAQSP RCLININTLV EDKGKLFGVE
121IEIIDINDNN PKFQVEDLEV KINEIAVPGA RYPLPEAVDP DVGVNSLQSY QLSPNHHFSL
181DVQTGDNGAI NPELVLERAL DREEEAAHHL VLTASDGGKP PRSSTVRIHV TVLDTNDNAP
241VFPHPIYRVK VLENMPPGTR LLTVTASDPD EGINGKVAYK FRKINEKQTP LFQLNENTGE
301ISIAKSLDYE ECSFYEMEIQ AEDVGALLGR TKLLISVEDV NDNRPEVIIT SLFSPVLENS
361LPGTVIAFLS VHDQDSGKNG QVVCYTRDNL PFKLEKSIGN YYRLVTRKYL DRENVSIYNI
421TVMASDLGTP PLSTETQIAL HVADINDNPP TFPHASYSAY ILENNLRGAS IFSLTAHDPD
481SQENAQVTYS VTEDTLQGAP LSSYISINSD TGVLYALQSF DYEQIRDLQL LVTASDSGDP
541PLSSNMSLSL FVLDQNDNAP EILYPALPTD GSTGVELAPR SAERGYLVTK VVAVDRDSGQ
601NAWLSYRLLK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAVQDHG QPPLSATVTL
661TVAVADSIPE VLTELGSLKP SVDPNDSSLT LYLVVAVAAI SCVFLAFVAV LLGLRLRRWH
721KSRLLQDSGG RLVGVPASHF VGVEEVQAFL QTYSQEVSLT ADSRKSHLIF PQPNYADMLI
781SQEGCEKNDS LLTSVDFHEY KNEADHGQQA PPNTDWRFSQ AQRPGTSGSQ NGDDTGTWPN
841NQFDTEMLQA MILASASEAA DGSSTLGGGA GTMGLSARYG PQFTLQHVPD YRQNVYIPGS
901NATLTNAAGK RDGKAPAGGN GNKKKSGKKE KK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.