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PCDHGA6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGA6 antibodies →
$725.00each
Catalog Number: LS-G111611-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA6 (NM_018919), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDHGA6 | Protocadherin gamma-A6 | PCDH-GAMMA-A6
Origin Species
Human
NCBI GenBank
NM_018919
SwissProt
Q9Y5G7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPQRHPQR SEQVLLLTLL GTLWGAAAAQ IRYSIPEELE KGSFVGNIVK DLGLEPQELA
61EHGVRIVSRG RMQLFSLNPR NGSLVTAGRI DREELCAQSP RCLVSFNILV EDKLNLYPVE
121VEIVDINDNT PRFLKEELEV KILENAAPSS RFPLMEVYDP DVGMNSLQGF KLSGNSHFSV
181DVQSEAHGPK YPELVLEGTL DREGEAVYRL VLTAMDGGDP VRSSVAQILV TVLDVNDNTP
241MFTQPVYRVS VPENLPVGTP VLAVTATDQD EGVHGEVTYS FVKITEKISQ IFCLNVLTGE
301ISTSANLDYE DSSFYELGVE ARDGPGLRDR AKVLITILDV NDNVPEVVVT SGSRTIAESA
361PPGTVIALFQ VFDRDSGLNG LVTCSIPRSL PFELEKSVGN YYRLVTNAAL DREEVFLYNI
421TVTATDKGTP PLSTETIISL NVADTNDNPP TFPHSSYSVY VLENNPRGAS IFSVNALDPD
481VDQNAQVSYS LAEDTLQGAP LSSYVSINSD TGILYALRSF DYEQLRDLQL WVTASDSGDP
541PLSSNVSLSL FVLDQNDNAP EILYPALPTD GSTGVELAPR SAEPGYLVTK VVAVDRDSGQ
601NAWLSYRLLK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAVQDHG QPPLSATVTL
661TVAVADRIPD ILADLGSLEP SAKPNDSDLT LYLVVAVAAV SCVFLAFVIV LLALRLQRWH
721KSRLLQASGG GLASMPGSHF VGVEGVRAFL QTYSHEVSLT ADSRKSHLIF PQPNYADTLI
781NQESYEKSEP LLITQDLLET KGEPRQLQQA PPNTDWRFSQ AQRPGTSGSQ NGDDTGTWPN
841NQFDTEMLQA MILASASEAA DGSSTLGGGA GTMGLSARYG PQFTLQHVPD YRQNVYIPGS
901NATLTNAAGK RDGKAPAGGN GNKKKSGKKE KK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.