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PCDHGA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111610-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA5 (NM_018918), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDH-GAMMA-A5 | PCDHGA5 | PCDH-GAMMA-A5 | Protocadherin gamma-A5 | Cadherin ME3
Origin Species
Human
NCBI GenBank
NM_018918
SwissProt
Q9Y5G8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASPPRGWGC GELLLPFMLL GTLCEPGSGQ IRYSMPEELD KGSFVGNIAK DLGLEPQELA
61ERGVRIVSRG RTQLFALNPR SGSLVTAGRI DREELCAQSP LCVVNFNILV ENKMKIYGVE
121VEIIDINDNF PRFRDEELKV KVNENAAAGT RLVLPFARDA DVGVNSLRSY QLSSNLHFSL
181DVVSGTDGQK YPELVLEQPL DREKETVHDL LLTALDGGDP VLSGTTHIRV TVLDANDNAP
241LFTPSEYSVS VPENIPVGTR LLMLTATDPD EGINGKLTYS FRNEEEKISE TFQLDSNLGE
301ISTLQSLDYE ESRFYLMEVV AQDGGALVAS AKVVVTVQDV NDNAPEVILT SLTSSISEDC
361LPGTVIALFS VHDGDSGENG EIACSIPRNL PFKLEKSVDN YYHLLTTRDL DREETSDYNI
421TLTVMDHGTP PLSTESHIPL KVADVNDNPP NFPQASYSTS VTENNPRGVS IFSVTAHDPD
481SGDNARVTYS LAEDTFQGAP LSSYVSINSD TGVLYALRSF DYEQLRDLQL WVTASDSGNP
541PLSSNVSLSL FVLDQNDNTP EILYPALPTD GSTGVELAPR SAEPGYLVTK VVAVDKDSGQ
601NAWLSYRLLK ASEPGLFAVG LHTGEVRTAR ALLDRDALKQ SLVVAVEDHG QPPLSATFTV
661TVAVADRIPD ILADLGSIKT PIDPEDLDLT LYLVVAVAAV SCVFLAFVIV LLVLRLRRWH
721KSRLLQAEGS RLAGVPASHF VGVDGVRAFL QTYSHEVSLT ADSRKSHLIF PQPNYADTLL
781SEESCEKSEP LLMSDKVDAN KEERRVQQAP PNTDWRFSQA QRPGTSGSQN GDDTGTWPNN
841QFDTEMLQAM ILASASEAAD GSSTLGGGAG TMGLSARYGP QFTLQHVPDY RQNVYIPGSN
901ATLTNAAGKR DGKAPAGGNG NKKKSGKKEK K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

931 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.