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PCDHGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111608-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA3 (NM_018916), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDHGA3 | PCDH-GAMMA-A3 | Protocadherin gamma-A3
Origin Species
Human
NCBI GenBank
NM_018916
SwissProt
Q9Y5H0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTNCLSFRNG RGLALLCALL GTLCETGSGQ IRYSVSEELD KGSFVGNIAN DLGLEPRELA
61ERGVRIVSRG RTQLFSLNPQ SGSLVTAERI DREELCAQIP LCLVKINILV EDKLKIFEVE
121IEIKDINDNA PNFPTEELEI KIGELTVPGT RFPIKTAFDP DVGINSLQNY KLSPNDYFSL
181AVNSVSEGAK YPELVLERAL DREKKEIHQL VLVASDGGDP VHSGNLHIQV IVLDANDNPP
241MFTQPEYRVS VWENVPVGTR LLTVNATDPD EGFNAQVSYI LDKMPGKIAE IFHLNSVSGE
301VSILKSLDYE DAMFYEIKIE AQDGPGLLSR AKILVTVLDV NDNAPEITIT SLTSSVPEEG
361TVGREIALID VHDRDSGQNG QVEVFVLGNL PFKLEKSIDQ YYRLVTATSL DREQISEYNI
421SLRASDGGSP PLSTETHITL HVIDINDNPP TFPHLSYSAY IPENNPRGAS IFSVTAQDPD
481SNNNARITYA LTEDTLQGAP LSSFVSINSN TGVLYALRSF DYEQFRDLKL LVTASDSGNP
541PLSSNVSLNL FVLDQNDNAP EILYPALPTD GSTGVELAPR SAEPGYLVTK VVAVDRDSGQ
601NAWLSYRLLK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAVQDHG QPPLSATVTL
661TVAVADRIPD ILADLGSLEP SAKPNDSDLT LYLVVAVAAV SCVFLAFVIV LLALRLRRWH
721KSRLLQASGG GLASTPGSHF VGADGVRAFL QTYSHEVSLT ADSRKSHLIF PQPNYADTLI
781SQESCEKSEP LLITQDLLEM KGDSNLLQQA PPNTDWRFSQ AQRPGTSGSQ NGDDTGTWPN
841NQFDTEMLQA MILASASEAA DGSSTLGGGA GTMGLSARYG PQFTLQHVPD YRQNVYIPGS
901NATLTNAAGK RDGKAPAGGN GNKKKSGKKE KK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.