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PCDHGA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHGA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHGA2 antibodies →
$504.00each
Catalog Number: LS-G109099-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHGA2 (NM_032009), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDHGA2 | PCDH-GAMMA-A2 | Protocadherin gamma-A2
Origin Species
Human
NCBI GenBank
NM_018915
SwissProt
Q9Y5H1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAALQKLPHC RKLVLLCFLL ATLWEARAGQ IRYSVREEID RGSFVGNIAK DLGLEPLALA
61EQGVRIVSRG RSQLFALNPR SGSLVTANRI DREELCAQSA PCLLNFNILL EDKLTIYSVE
121VEITDINDNA PRFGVEELEL KISETTTPGF RIPLKNAHDA DVGENALQKY ALNPNDHFSL
181DVRRGADGNK YPELVLERSL DREEEAVHHL VLVASDGGDP VLSGTSRICV KVLDANDNAP
241VFTQPEYRIS IPENTLVGTR ILTVTATDAD EGYYAQVVYF LEKSPGETSE VFELKSTSGE
301LTIIKDLDYE DATFHEIDIE AQDGPGLLTR AKVIVTVLDV NDNAPEFYMT SATSSVSEDS
361LPGTIIGLFN VHDRDSGQNA FTTCSLPEDL PFKLEKSVDN YYRLVTTRAL DREQFSFYNI
421TLTAKDGGNP SLSTDAHILL QVADINDNAP AFSRTSYSTY IPENNPRGAS VFSVTAHDPD
481SNDNAHVTYS FAEDTVQGAP LSSYISINSD TGVLYALRSF DYEQLRDLQV WVIARDSGNP
541PLSSNVSLSL FVLDQNDNAP EILYPAFPTD GSTGVELAPR SAEPGYLVTK VVAVDRDSGQ
601NAWLSYHLLK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAIQDHG QPPLSATVTL
661TVAVADRIPD ILADLGSLEP SAIPNDSDLT LYLVVAVAAV SCVFLAFVIV LLAHRLRRWH
721KSRLLQASGG SLTGMQSSHF VGVDGVRAFL QTYSHEVSLT ADSRKSHLIF PQPNYADTLI
781SQESCEKKDF LSAPQSLLEE EREETFSQVI YLFTTYVLAS LLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

823 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.