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PCDHB8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111376-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB8 (NM_019120), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH3I | PCDHB8 | Protocadherin-3i | Protocadherin beta-8 | PCDH-BETA8 | Protocadherin beta 8 | PCDH-beta-8
Origin Species
Human
NCBI GenBank
NM_019120
SwissProt
Q9UN66

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEASGKLICR QRQVLFSFLL LGLSLAGAAE PRSYSVVEET EGSSFVTNLA KDLGLEQREF
61SRRGVRVVSR GNKLHLQLNQ ETADLLLNEK LDREDLCGHT EPCVLRFQVL LESPFEFFQA
121ELQVIDINDH SPVFLDKQML VKVSESSPPG TAFPLKNAED LDIGQNNIEN YIISPNSYFR
181VLTRKRSDGR KYPELVLDKA LDREEEAELR LTLTALDGGS PPRSGTAQVY IEVVDVNDNA
241PEFEQPFYRV QISEDSPISF LVVKVSATDV DTGVNGEISY SLFQASDEIS KTFKVDFLTG
301EIRLKKQLDF EKFQSYEVNI EARDAGGFSG KCTVLIQVID VNDHAPEVTM SAFTSPIPEN
361APETVVALFS VSDLDSGENG KISCSIQEDL PFLLKSSVGN FYTLLTETPL DRESRAEYNV
421TITVTDLGTP RLTTHLNMTV LVSDVNDNAP AFTQTSYTLF VRENNSPALH IGSVSATDRD
481SGTNAQVTYS LLPPQDPHLP LASLVSINTD NGHLFALRSL DYEALQAFEF RVGASDRGSP
541ALSSEALVRV LVLDANDNSP FVLYPLQNGS APCTELVPRA AEPGYLVTKV VAVDGDSGQN
601AWLSYQLLKA TEPGLFGVWA HNGEVRTARL LSERDAAKQR LVVLVKDNGE PPCSATATLH
661LLLVDGFSQP YLPLPEAAPA QGQADSLTVY LVVALASVSS LFLFSVLLFV AVLLCRRSRA
721ASVGRCSVPE GPFPGHLVDV RGTGSLSQNY QYEVCLAGGS GTNEFQFLKP VLPNIQGHSF
781GPEMEQNSNF RNGFGFSLQL K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

801 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.