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PCDHB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101598-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB6 (NM_018939), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDHB6 | PCDH-BETA6 | Protocadherin beta-6 | Protocadherin beta 6 | PCDH-beta-6
Origin Species
Human
NCBI GenBank
AF217752
SwissProt
Q9Y5E3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMQTKVQNKK RQVAFFILLM LWGEVGSESI QYSVLEETES GTFVANLTKD LGLRVGELAS
61RGARVVFKGN RQHLQFDPQT HDLLLNEKLD REELCGSTEP CVLPFQVLLE NPLQFFQASL
121RVRDINDHAP EFPAREMLLK ISEITMPGKI FPLKMAHDLD TGSNGLQRYT ISSNPHFHVL
181TRNRSEGRKF PELVLDKPLD REEQPQLRLT LIALDGGSPP RSGTSEIQIQ ILDINDNVPE
241FAQELYEAQV PENNPLGSLV ITVSARDLDA GSFGKVSYAL FQVDDVNQPF EINAITGEIR
301LRKALDFEEI QSYDVDVEAT DGGGLSGKCS LVVRVLDVND NAPELTMSFF ISLIPENLPE
361ITVAVFSVSD ADSGHNQQVI CSIENNLPFL LRPSVENFYT LVTEGALDRE SRAEYNITIT
421VTDLGTPRLK TQQSITVQVS DVNDNAPAFT QTSYTLFVRE NNSPALHIGS VSATDRDSGI
481NAQVTYSLLP PQDPHLPLSS LVSINADNGH LFALRSLDYE ALQSFEFRVG ATDRGSPALS
541SEALVRLLVL DANDNSPFVL YPLQNGSAPC TELVPRAAEP GYLVIKVVAV DGDSGQNAWL
601SYQLLKATEL GLFGVWAHNG EVRTARLLSE RDAAKQRLVV LVKDNGEPPR SATATLHVLL
661VDGFSQPYLP LPEAAPAQAQ ADSLTVYLVV ALASVSSLFL FSVLLFVAVR LCRRSRAASV
721GRYSVPEGPF PGHLVDVSGT GTLSQSYQYK VCLTGGSETN EFKFLKPIMP NFPPQGTERE
781MEETPTSRNS FPFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

794 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.