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PCDHB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113030-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB5 (NM_015669), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-BETA5 | PCDHB5 | Protocadherin beta-5 | PCDH-beta-5 | Protocadherin beta 5
Origin Species
Human
NCBI GenBank
NM_015669
SwissProt
Q9Y5E4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.43 kDa
Protein Type
Lysate
Amino Acid Sequence
1METALAKTPQ KRQVMFLAIL LLLWEAGSEA VRYSIPEETE SGYSVANLAK DLGLGVGELA
61TRGARMHYKG NKELLQLDIK TGNLLLYEKL DREVMCGATE PCILHFQLLL ENPVQFFQTD
121LQLTDINDHA PEFPEKEMLL KIPESTQPGT VFPLKIAQDF DIGSNTVQNY TISPNSHFHV
181ATHNRGDGRK YPELVLDKAL DREERPELSL TLTALDGGAP PRSGTTTIRI VVLDNNDNAP
241EFLQSFYEVQ VPENSPLNSL VVVVSARDLD AGAYGSVAYA LFQGDEVTQP FVIDEKTAEI
301RLKRALDFEA TPYYNVEIVA TDGGGLSGKC TVAIEVVDVN DNAPELTMST LSSPTPENAP
361ETVVAVFSVS DPDSGDNGRM ICSIQNDLPF LLKPTLKNFY TLVTQRTLDR ESQAEYNITI
421TVTDMGTPRL KTEHNITVLV SDVNDNAPAF TQTSYTLFVR ENNSPALHIG SVSATDRDSG
481TNAQVTYSLL PPQNPHLRLA SLVSINADNG HLFALRSLDY EALQAFEFRV GATDRGSPAL
541SSEALVRVLV LDANDNSPFV LYPLQNGSAP CTELVPRAAE PGYLVTKVVA VDGDSGQNAW
601LSYQLLKATE PGLFSMWAHN GEVRTARLLS ERDAAKHRLV VLVKDNGEPP RSATATLHVL
661LVDGFSQPYL PLPEAAPAQA QADSLTVYLV VALASVSSLF LFSVLLFVAV RLCRRSRAAP
721VGRCSVPEGP FPGHLVDVSG TGTLSQSYHY EVCLTGDSGA GEFKFLKPII PNLLPQGAGE
781EIGKTAAFRN SFGLN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

795 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.