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PCDHB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111629-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Protocadherin beta 3(PCDHB3) (NM_018937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Protocadherin beta-3 | Protocadherin beta 3 | PCDH-beta-3 | PCDHB3 | PCDH-BETA3
Origin Species
Human
NCBI GenBank
NM_018937
SwissProt
Q9Y5E6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAGGERFLR QRQVLLLFVF LGGSLAGSES RRYSVAEEKE RGFLIANLAK DLGLRVEELA
61ARGAQVVSKG NKQHFQLSHQ TGDLLLNEKL DREELCGPTE PCILHFQILL QNPLQFVTNE
121LRIIDVNDHS PVFFENEMHL KILESTLPGT VIPLGNAEDL DVGRNSLQNY TITPNSHFHV
181LTRSRRDGRK YPELVLDKAL DREEQPELSL TLTALDGGSP PRSGTAQINI QVLDINDNAP
241EFAQPLYEVA VLENTPVNSV IVTVSASDLD TGSFGTISYA FFHASEEIRK TFQLNPITGD
301MQLVKYLNFE AINSYEVDIE AKDGGGLSGK STVIVQVVDV NDNPPELTLS SVNSPIPENS
361GETVLAVFSV SDLDSGDNGR VMCSIENNLP FFLKPSVENF YTLVSEGALD RETRSEYNIT
421ITITDLGTPR LKTKYNITVL VSDVNDNAPA FTQTSYTLFV RENNSPALHI GSVSATDRDS
481GTNAQVTYSL LPPQDPHLPL SSLVSINADN GHLFALRSLD YEALQAFEFR VGAADRGSPA
541LSSEALVRVL VLDANDNSPF VLYPLQNGSA PCTELVPRAA EPGYLVTKVV AVDSDSGQNA
601WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDAAKHRL VVLVKDNGEP PRSATATLHV
661LLVDGFSQPY LPLPEAAPAQ AQADLLTVYL VVALASVSSL FLFSVLLFVA VRLCRRSRAA
721SVGRCSVPEG PFPGQMVDVS GTGTLSQSYQ YEVCLTGGSG TNEFKFLKPI IPNFVAQGAE
781RVSEANPSFR KSFEFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

796 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.