Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PCDHB2 / Protocadherin Beta 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111628-100
Catalog Number: LS-G111628
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB2 (NM_018936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PcdhbB | Protocadherin beta 2 | PCDH-beta-2 | PCDHB2 | PCDH-BETA2 | Protocadherin beta-2
- Origin Species
- Human
- NCBI GenBank
- NM_018936
- SwissProt
- Q9Y5E7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAGEGKERV PKQRQVLIFF VLLGIAQASC QPRHYSVAEE TESGSFVANL LKDLGLEIGE61LAVRGARVVS KGKKMHLQFD RQTGDLLLNE KLDREELCGP TEPCVLPFQV LLENPLQFFQ121AELRIRDVND HSPVFLDKEI LLKIPESITP GTTFLIERAQ DLDVGTNSLQ NYTISPNFHF181HLNLQDSLDG IILPQLVLNR ALDREEQPEI RLTLTALDGG SPPRSGTALV RIEVVDINDN241VPEFAKLLYE VQIPEDSPVG SQVAIVSARD LDIGTNGEIS YAFSQASEDI RKTFRLSAKS301GELLLRQKLD FESIQTYTVN IQATDGGGLS GTCVVFVQVM DLNDNPPELT MSTLINQIPE361NLQDTLIAVF SVSDPDSGDN GRMVCSIQDD LPFFLKPSVE NFYTLVISTA LDRETRSEYN421ITITVTDFGT PRLKTEHNIT VLVSDVNDNA PAFTQTSYTL FVRENNSPAL HIGSVSATDR481DSGTNAQVTY SLLPPQDPHL PLASLVSINA DNGHLFALQS LDYEALQAFE FRVGATDRGS541PALSSEALVR VLVLDANDNS PFVLYPLQNG SAPCTELVPR AAEPGYLVTK VVAVDGDSGQ601NAWLSYQLLK ATEPGLFGVW AHNGEVRTAR LLRERDAAKQ RLVVLVKDNG EPPRSATATL661HVLLVDGFSQ PYLLLPEAAP AQAQADLLTV YLVVALASVS SLFLFSVLLF VAVRLCRRSR721AASVGRCSVP EGPFPGQMVD VSGTGTLSQS YQYEVCLTGG SGTNEFKFLK PIIPNFVAQG781AERVSEANPS FRKSFEFTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
798 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.