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PCDHB16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHB16 antibodies →
$504.00each
Catalog Number: LS-G110866-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB16 (NM_020957), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1621 | PCDH-beta-16 | PCDHB16 | PCDH-BETA16 | PCDHB8a | Protocadherin beta 16 | Protocadherin beta-16 | PCDHbeta 16 | Protocadherin beta 8a | Protocadherin-3x | Cadherin ME1 | PCDH3X
Origin Species
Human
NCBI GenBank
NM_020957
SwissProt
Q9NRJ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEIGWMHNRR QRQVLVFFVL LSLSGAGAEL GSYSVVEETE RGSFVANLGK DLGLGLTEMS
61TRKARIISQG NKQHLQLKAQ TGDLLINEKL DREELCGPTE PCILHFQVLM ENPLEIFQAE
121LRVIDINDHS PMFTEKEMIL KIPENSPLGT EFPLNHALDL DVGSNNVQNY KISPSSHFRV
181LIHEFRDGRK YPELVLDKEL DREEEPQLRL TLTALDGGSP PRSGTAQVRI EVVDINDNAP
241EFEQPIYKVQ IPENSPLGSL VATVSARDLD GGANGKISYT LFQPSEDISK TLEVNPMTGE
301VRLRKQVDFE MVTSYEVRIK ATDGGGLSGK CTLLLQVVDV NDNPPQVTMS ALTSPIAENS
361PEIVVAVFSV SDPDSGNNGK TISSIQEDLP FLLKPSVKNF YTLVTERALD REARAEYNIT
421LTVTDMGTPR LKTEHNITVQ ISDVNDNAPT FTQTSYTLFV RENNSPALHI GSVSATDRDS
481GTNAQVTYSL LPPQDPHLPL ASLVSINADN GHLFALRSLD YEALQAFEFR VGATDRGSPA
541LSREALVRVL VLDANDNSPL VLYPLQNGSA PCTELVPRAA EPGYLVTKVV AVDGDSGQNA
601WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDAAKQRL VVLVKDNGEP PRSATATLHV
661LLVDGFSQPF LPLPEAAPGQ TQANSLTVYL VVALASVSSL FLFSVLLFVA VRLCRRSRAA
721SVGRCSMPEG PFPGRLVDVS GTGTLSQSYQ YEVCLTGGSE TSEFKFLKPI IPNFSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

776 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.