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PCDHB14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111625-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB14 (NM_018934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDHB14 | PCDH-beta-14 | PCDH-BETA14 | Protocadherin beta 14 | Protocadherin beta-14
Origin Species
Human
NCBI GenBank
NM_018934
SwissProt
Q9Y5E9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEIRGALDLR KRQVLIFLVL LGLSRAGTES AHYSVAEETE IGSFVANLAR DLGLGVEELS
61SREARVVSDD NKKYLHLDLL TGNLLLNEKL DRDELCGSTE PCVLHFQVVL ENPLQFFRFE
121LCVKDINDHS PTFLDKEILI KISEGTTVGA TFLMESAQDL DVGSNSLQNY TISPNSHFYI
181KIPDSSDRKI YPELVLDRAL DYEQEAELRL TLTAVDGGSP PKSGTTLVLI KVLDINDNAP
241EFPQSLYEVQ VPEDRPLGSW IATISAKDLD AGNYGKISYT FFHASEDIRK TFEINPISGE
301VNLRSPLDFE VIQSYTINIQ ATDGGGLSGK CTLLVKVMDI NDNPPEVTIS SITKRIPENA
361SETLVALFSI LDQDSGDNGR MICSIQDNLP FFLKPTFKNF FTLVSEKALD RESQAEYNIT
421ITVTDLGTPR LKTEYNITVL LSDVNDNAPT FTQTSYTLFV RENNSPALHI GSVSATDRDS
481GTNAQVNYSL LPPQDRHLPL ASLVSINADN GHLFALRSLD YEALQEFEFR VGATDRGSPA
541LSSEALVRVL VLDANDNSPF VLYPLQNGSA PCTELVPRAA EPGYLVTKVV AVDGDSGQNA
601WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDAAKHRL VVLVKDNGEP PRSATATLHV
661LLVDGFSQPY LPLPEAAPAQ AQADSLTVYL VVALASVSSL FLFSVLLFVA VRLCRRSRAA
721SVGRCSVPEG PFPGHLVDVS GTGTLSQSYQ YEVCLTGGSG TNEFKFLKPI IPNFQVHDTG
781RNMGEIENFR NSFGLNIQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

798 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.