LSBio
PCDHB12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHB12 antibodies →
$504.00each
Catalog Number: LS-G111623-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Protocadherin beta 12(PCDHB12) (NM_018932), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-BETA12 | PCDH-beta-12 | PCDHB12 | Protocadherin beta-12 | Protocadherin beta 12
Origin Species
Human
NCBI GenBank
AF217746
SwissProt
Q9Y5F1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENGGAGTLQ IRQVLLFFVL LGMSQAGSET GNFLVMEELQ SGSFVGNLAK TLGLEVSELS
61SRGARVVSND NKECLQLDTN TGDLLLREML DREELCGSNE PCVLYFQVLM KNPTQFLQIE
121LQVRDINDHS PVFLEKEMLL EIPENSPVGA VFLLESAKDL DVGINAVKSY TINPNSHFHV
181KIRVNPDNRK YPELVLDKAL DYEERPELSF ILTALDGGSP PRSGTALVRV VVVDINDNSP
241EFEQAFYEVK ILENSILGSL VVTVSAWDLD SGTNSELSYT FSHASEDIRK TFEINQKSGD
301ITLTAPLDFE AIESYSIIIQ ATDGGGLFGK STVRIQVMDV NDNAPEITVS SITSPIPENT
361PETVVMVFRI RDRDSGDNGK MVCSIPEDIP FVLKSSVNNY YTLETERPLD RESRAEYNIT
421ITVTDLGTPR LKTEHNITVL VSDVNDNAPA FTQTSYALFV RENNSPALHI GSISATDRDS
481GTNAQVNYSL LPSQDPHLPL ASLVSINADN GHLFALRSLD YEALQGFQFR VGATDHGSPA
541LSSEALVRVL VLDANDNSPF VLYPLQNGSA PCTELVPWAA EPGYLVTKVV AVDGDSGQNA
601WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDAAKHRL VVLVKDNGEP PRSATATLHV
661LLVDGFSQPY LPLPEAAPAQ AQADSLTVYL VVALASVSSL FLFSVLLFVA VRLCRRSRAA
721PVGRCSVPEG PFPGHLVDVS GTGTLSQSYH YEVCVTGGSR SNKFKFLKPI IPNFLPQSTG
781SEVEENPPFQ NNLGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

795 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.