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PCDHB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114311-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB1 (NM_013340), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-BETA1 | Protocadherin beta 1 | Protocadherin beta-1 | PCDH-beta-1 | PCDHB1
Origin Species
Human
NCBI GenBank
NM_013340
SwissProt
Q9Y5F3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.49 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGTRRKSLQ NRQVGSLLIF LCISVGDATT IRYSVAEEME SGSFVANVAK DLGLEVGKLA
61ARGARLVSEG NKMHFRLHRK TGDLFVKEKL DRESLCGKAD PCVLHFEVVL VEPLQSFRAE
121VRVFDINDNA PVFLNKEPLL KIPESTPLGS RFPLQSAQDL DVGLNGLQNY TLSANGYFHL
181HTRFCSHGPK YAELVLNKPL DREEQPEVNL TITAVDGGSP PKSGTAHIHV VVLDVNDHVP
241QFSRLVYRAQ VSENSPNGSL VATVTAVDLD EGTNKAITYS LAQNPEAILK TFQIDPQNGE
301VRLRGPLDFE AIETYDIDIQ ATDGGGLSAH SKVLVEVVDV NDNPPEVMVS SVSSPLPEDS
361PPQTVVALFT IRDRDIRVGG KVTCFLREDL PFVIKPTFGN SYSLVTDRSL DREEVSGYNI
421TIVAMDTGPP SLSAETMIEV LISDVNDNPP IFREDSYILT VRENNSPAVF IGKVHAEDLD
481LGENAQITYS LLPPKNGDLS VFAYISINSG NGKLYALRTM DYEAIQDFQF VVKATDGGFL
541SLSSQVTVRV VVLDDNDNRP MILYPLQNGT LPCNDLVPRS AEAGYLVTKV VAVDGDSGQN
601SWLSYHLLKA TDLGLFSVQR QNGEIHTLRQ ISERDPMMQK LIILVQDHGQ PALSTTVSLN
661ILLVDGFSEP YLQFQDPTKH SRKVNPSTKY LVISLVILSF LFLLSVIVIF IIHVYQKIKY
721REKFTIQEHF YDDCNFSNNL VQGQGNGSLS RPCPYEMCSA TGTGNSEFRF LKRFMPNFPF
781PHATGEIKME AGSSLPPNSD RNKSQRLEGH DQVSDDYM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

818 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.