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PCDHA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHA13 antibodies →
$725.00each
Catalog Number: LS-G111596-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHA13 (NM_018904), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRNR5 | CNR5 | CNRS5 | KIAA0345-like 1 | Protocadherin alpha 13 | Protocadherin alpha-13 | PCDH-alpha-13 | CNRN5 | Ortholog of mouse CNR5 | PCDH-ALPHA13 | PCDHA13
Origin Species
Human
NCBI GenBank
NM_018904
SwissProt
Q9Y5I0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSSWQGGPR PRQLLLWLLI LAAWETGSGQ LHYSVPEEAK HGTFVGRIAQ DLGLELAELV
61PRLFRVASKR HGDLLEVNLQ NGILFVNSRI DREELCGRSA ECSIHLEVIV DRPLQVFHVE
121VKVRDINDNP PIFPESKKRI IIAESRPPET RFPLDGASDA DIGVNSALTY RLDPNDYFTL
181DAQNSLEQMS SLSLVLRKTL DREEIQEHSL LLTASDGGKP ELTGTVQLLI TILDVNDNAP
241EFYQSVYKVT VLENAFNGTL VIKLNATDPD DGTNGDIVYS FRRPVWPAVV YAFTINPNNG
301EIRTKGKLDF EEKKLYEISV EAVDKGNIPM AGHCTLLVEV LDVNDNAPEV TITSLSLPIR
361EDTQPSAIIA LISVSDRDSG SNGQVTCTLT PHVPFKLVST YKNYYSLVLD SALDRESVSA
421YELVVTARDG GSPSLWATAS VSVGVADVND NAPAFAQPEY TVFVKENNPP GCHIFTVSAQ
481DADAQENALV SYSLVERRVG ERALSSYVSV HAESGKVYAL QPLDHEELEL LQFQVSARDS
541GVPPLGSNVT LQVFVLDEND NAPALLTPGA GSAGGTVSEL MPRSVGAGHV VAKVRAVDAD
601SGYNAWLSYE LQLAAVGARI PFRVGLYTGE ISTTRPLDEV DAPHHRLLVL VKDHGEPALT
661ATATVLLSLV ESGQAPQASS RASAGAVGPE AALVDVNVYL IIAICAVSSL LVLTLLLYTA
721LRCSAPPTEG ACAPGKPTLV CSSAAGSWSY SQQRRPRVCS GEGPHKTDLM AFSPSLPPCL
781GSAEGTGQRE EDSECLKEPR QPNPDWRYSA SLRAGMHSSV HLEEAGILRA GPGGPDQQWP
841TVSSATPEPE AGEVSPPVGA GVNSNSWTFK YGPGNPKQSG PGELPDKFII PGSPAIISIR
901QEPTNSQIDK SDFITFGKKE ETKKKKKKKK GNKTQEKKEK GNSTTDNSDQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

950 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.