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PCDHA11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHA11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHA11 antibodies →
$725.00each
Catalog Number: LS-G111594-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHA11 (NM_018902), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRNR7 | CNRS7 | CNR7 | CNRN7 | KIAA0345-like 3 | Ortholog of mouse CNR7 | PCDHA11 | Protocadherin alpha 11 | Protocadherin alpha-11 | PCDH-alpha-11 | PCDH-ALPHA11
Origin Species
Human
NCBI GenBank
NM_018902
SwissProt
Q9Y5I1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGFQRRGLG TPRLQLWLLL LEFWEVGSGQ LHYSVSEEAK HGTFVGRIAQ DLGLELAELV
61QRLFRVASKT HGDLLEVNLQ NGILFVNSRI DREELCGQSA ECSIHLEVIV DRPLQVFHVN
121VEVKDINDNP PVFSLREQKL LIAESKQSDS RFPLEGASDA DIEENALLTY RLSKNEYFSL
181DSPTNGKQIK RLSLILKKSL DREKTPELNL LLTATDGGKP ELTGTVRLLV QVLDVNDNDP
241EFDKSEYKVS LMENAAKETL VLKLNATDRD EGVNGEVTYS LMSIKPNGRH LFTLDQNNGE
301VRVNGTLDYE ENKFYKIEVQ ATDKGTPPMA GHCTVWVEIL DTNDNSPEVA VTSLSLPVRE
361DAQPSTVIAL ISVSDRDSGV NGQVTCSLTP HVPFKLVSTF KNYYSLVLDS ALDRENVWAY
421ELVVTARDGG SPSLWATARV SVEVADVNDN APAFAQPEYT VFVKENNPPG CHIFTVSARD
481ADAQENALVS YSLVERRLGD RALSSYVSVH AESGKVYALQ PLDHEELELL QFQVSARDAG
541VPPLSSNVTL QVFVLDENDN APALLATQAG SAGGAVNKLV PRSVGAGHVV AKVRAVDADS
601GYNAWLSYEL QPAAGGSRIP FRVGLYTGEI STTRALDEAD SPRHRLLVLV KDHGEPALTA
661TATVLVSLVE SGQAPKASSR TLAGAASPEA ALVDVNVYLI IAICVVSSLL VLTLLLYTAL
721WWSATPTEGA CAPGKPTLVC SRAVGSWSYS QQRRQRVCSE EGPPKTDLMA FSPSLPLGLN
781KEEEGERQEP GSNHPGQPRQ PNPDWRYSAS LRAGMHSSVH LEEAGILRAG PGGPDQQWPT
841VSSATPEPEA GEVSPPVGAG VNSNSWTFKY GPGNPKQSGP GELPDKFIIP GSPAIISIRQ
901EPTNSQIDKS DFITFGKKEE TKKKKKKKKG NKTQEKKEKG NSTTDNSDQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

949 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.