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PCDHA10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDHA10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDHA10 antibodies →
$725.00each
Catalog Number: LS-G109159-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHA10 (NM_031860), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNRN8 | PCDHA10 | PCDH-ALPHA10 | Protocadherin alpha-10 | PCDH-alpha-10 | CNR8 | CNRS8 | CRNR8 | KIAA0345-like 4 | Ortholog to mouse CNR8 | Protocadherin alpha 10
Origin Species
Human
NCBI GenBank
NM_018901
SwissProt
Q9Y5I2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVSRCSCLGV QCLLLSLLLL AAWEVGSGQL HYSVYEEARH GTFVGRIAQD LGLELAELVQ
61RLFRVASKRH GDLLEVNLQN GILFVNSRID REELCGRSVE CSIHLEVIVD RPLQVFHVDV
121EVKDINDNPP RFSVTEQKLS IPESRLLDSR FPLEGASDAD VGENALLTYK LSPNEYFVLD
181IINKKDKDKF PVLVLRKLLD REENPQLKLL LTATDGGKPE FTGSVSLLIL VLDANDNAPI
241FDRPVYEVKM YENQVNQTLV IRLNASDSDE GINKEMMYSF SSLVPPTIRR KFWINERTGE
301IKVNDAIDFE DSNTYEIHVD VTDKGNPPMV GHCTVLVELL DENDNSPEVI VTSLSLPVKE
361DAQVGTVIAL ISVSDHDSGA NGQVTCSLTP HVPFKLVSTY KNYYSLVLDS ALDRERVSAY
421ELVVTARDGG SPPLWATASV SVEVADVNDN APAFAQSEYT VFVKENNPPG CHIFTVSAWD
481ADAQENALVS YSLVERRLGE RSLSSYVSVH AESGKVYALQ PLDHEELELL QFQPRQPNPD
541WRYSASLRAG MHSSVHLEEA GILRAGPGGP DQQWPTVSSA TPEPEAGEVS PPVGAGVNSN
601SWTFKYGPGN PKQSGPGELP DKFIIPGSPA IISIRQEPTN SQIDKSDFIT FGKKEETKKK
661KKKKKGNKTQ EKKEKGNSTT DNSDQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

685 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.