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PCDH9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103024-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH9 (NM_203487), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH9 | Protocadherin 9 | Protocadherin-9
Origin Species
Human
NCBI GenBank
NM_020403
SwissProt
Q9HC56

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLRDFYLLA ALIACLRLDS AIAQELIYTI REELPENVPI GNIPKDLNIS HINAATGTSA
61SLVYRLVSKA GDAPLVKVSS STGEIFTTSN RIDREKLCAG ASYAEENECF FELEVVILPN
121DFFRLIKIKI IVKDTNDNAP MFPSPVINIS IPENTLINSR FPIPSATDPD TGFNGVQHYE
181LLNGQSVFGL DIVETPEGEK WPQLIVQQNL DREQKDTYVM KIKVEDGGTP QKSSTAILQV
241TVSDVNDNRP VFKEGQVEVH IPENAPVGTS VIQLHATDAD IGSNAEIRYI FGAQVAPATK
301RLFALNNTTG LITVQRSLDR EETAIHKVTV LASDGSSTPA RATVTINVTD VNDNPPNIDL
361RYIISPINGT VYLSEKDPVN TKIALITVSD KDTDVNGKVI CFIEREVPFH LKAVYDNQYL
421LETSSLLDYE GTKEFSFKIV ASDSGKPSLN QTALVRVKLE DENDNPPIFN QPVIELSVSE
481NNRRGLYLTT ISATDEDSGK NADIVYQLGP NASFFDLDRK TGVLTASRVF DREEQERFIF
541TVTARDNGTP PLQSQAAVIV TVLDENDNSP KFTHNHFQFF VSENLPKYST VGVITVTDAD
601AGENKAVTLS ILNDNDNFVL DPYSGVIKSN VSFDREQQSS YTFDVKATDG GQPPRSSTAK
661VTINVMDVND NSPVVISPPS NTSFKLVPLS AIPGSVVAEV FAVDVDTGMN AELKYTIVSG
721NNKGLFRIDP VTGNITLEEK PAPTDVGLHR LVVNISDLGY PKSLHTLVLV FLYVNDTAGN
781ASYIYDLIRR TMETPLDRNI GDSSQPYQNE DYLTIMIAII AGAMVVIVVI FVTVLVRCRH
841ASRFKAAQRS KQGAEWMSPN QENKQNKKKK RKKRKSPKSS LLNFVTIEES KPDDAVHEPI
901NGTISLPAEL EEQSIGRFDW GPAPPTTFKP NSPDLAKHYK SASPQPAFHL KPDTPVSVKK
961HHVIQELPLD NTFVGGCDTL SKRSSTSSDH FSASECSSQG GFKTKGPLHT RQCNSHSKSD
1021NIPVTPQKCP SSTGFHIQEN EESHYESQRR VTFHLPDGSQ ESCSDSGLGD HEPVGSGTLI
1081SHPLPLVQPQ DEFYDQASPD KRTEADGNSD PNSDGPLGPR GLAEATEMCT QECLVLGHSD
1141NCWMPPGLGP YQHPKSPLST FAPQKEWVKK DKLVNGHTLT RAWKEDSNRN QFNDRKQYGS
1201NEGHFNNGSH MTDIPLANLK SYKQAGGATE SPKEHQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1237 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.