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PCDH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDH8 antibodies →
$725.00each
Catalog Number: LS-G108533-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH8 (NM_032949), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARCADLIN | PCDH8 | Protocadherin 8 | Protocadherin-8 | PAPC
Origin Species
Human
NCBI GenBank
NM_002590
SwissProt
O95206

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSPVRRWGSP CLFPLQLFSL CWVLSVAQSK TVRYSTFEED APGTVIGTLA EDLHMKVSGD
61TSFRLMKQFN SSLLRVREGD GQLTVGDAGL DRERLCGQAP QCVLAFDVVS FSQEQFRLVH
121VEVEVRDVND HAPRFPRAQI PVEVSEGAAV GTRIPLEVPV DEDVGANGLQ TVRLAEPHSP
181FRVELQTRAD GAQCADLVLL QELDRESQAA YSLELVAQDG GRPPRSATAA LSVRVLDAND
241HSPAFPQGAV AEVELAEDAP VGSLLLDLDA ADPDEGPNGD VVFAFGARTP PEARRLFRLD
301PRSGRLTLAG PVDYERQDTY ELDVRAQDRG PGPRAATCKV IVRIRDVNDN APDIAITPLA
361APGAPATSPF AAAAAAAALG GADASSPAGA GTPEAGATSL VPEGAARESL VALVSTSDRD
421SGANGQVRCA LYGHEHFRLQ PAYAGSYLVV TAASLDRERI AEYNLTLVAE DRGAPPLRTV
481RPYTVRVGDE NDNAPLFTRP VYEVSVRENN PPGAYLATVA ARDRDLGRNG QVTYRLLEAE
541VGRAGGAVST YVSVDPATGA IYALRSFDYE TLRQLDVRIQ ASDGGSPQLS SSALVQVRVL
601DQNDHAPVLV HPAPANGSLE VAVPGRTAKD TVVARVQARD ADEGANGELA FELQQQEPRE
661AFAIGRRTGE ILLTGDLSQE PPGRVFRALL VISDGGRPPL TTTATVSFVV TAGGGRGPAA
721PASAGSPERS RPPGSRLGVS GSVLQWDTPL IVIIVLAGSC TLLLAAIIAI ATTCNRRKKE
781PYGASPGFGK EPAPPVAVWK GHSFNTISGR EAEKFSGKDS GKGDSDFNDS DSDISGDALK
841KDLINHMQSG LWACTAECKI LGHSDRCWSP SCSGPNAHPS PHPPAQMSTF CKSTSLPRDP
901LRRDNYYQAQ LPKTVGLQSV YEKVLHRDYD RTVTLLSPPR PGRLPDLQEI GVPLYQSPPG
961RYLSPKKGAN ENV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

973 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.