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PCDH20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDH20 antibodies →
$725.00each
Catalog Number: LS-G129145-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH20 (NM_022843), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH13 | PCDH20 | Protocadherin 20 | Protocadherin-13 | Protocadherin-20 | Protocadherin 13
Origin Species
Human
NCBI GenBank
NM_022843
SwissProt
Q8N6Y1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGRGNARSS QALGVSWCPA TWHPRLDMGR LHRPRSSTSY RNLPHLFLFF LFVGPFSCLG
61SYSRATELLY SLNEGLPAGV LIGSLAEDLR LLPRSAGRPD PQSQLPERTG AEWNPPLSFS
121LASRGLSGQY VTLDNRSGEL HTSAQEIDRE ALCVEGGGGT AWSGSVSISS SPSDSCLLLL
181DVLVLPQEYF RFVKVKIAIR DINDNAPQFP VSQISVWVPE NAPVNTRLAI EHPAVDPDVG
241INGVQTYRLL DYHGMFTLDV EENENGERTP YLIVMGALDR ETQDQYVSII IAEDGGSPPL
301LGSATLTIGI SDINDNCPLF TDSQINVTVY GNATVGTPIA AVQAVDKDLG TNAQITYSYS
361QKVPQASKDL FHLDENTGVI KLFSKIGGSV LESHKLTILA NGPGCIPAVI TALVSIIKVI
421FRPPEIVPRY IANEIDGVVY LKELEPVNTP IAFFTIRDPE GKYKVNCYLD GEGPFRLSPY
481KPYNNEYLLE TTKPMDYELQ QFYEVAVVAW NSEGFHVKRV IKVQLLDDND NAPIFLQPLI
541ELTIEENNSP NAFLTKLYAT DADSEERGQV SYFLGPDAPS YFSLDSVTGI LTVSTQLDRE
601EKEKYRYTVR AVDCGKPPRE SVATVALTVL DKNDNSPRFI NKDFSFFVPE NFPGYGEIGV
661ISVTDADAGR NGWVALSVVN QSDIFVIDTG KGMLRAKVSL DREQQSSYTL WVEAVDGGEP
721ALSSTAKITI LLLDINDNPP LVLFPQSNMS YLLVLPSTLP GSPVTEVYAV DKDTGMNAVI
781AYSIIGRRGP RPESFRIDPK TGNITLEEAL LQTDYGLHRL LVKVSDHGYP EPLHSTVMVN
841LFVNDTVSNE SYIESLLRKE PEINIEEKEP QISIEPTHRK VESVSCMPTL VALSVISLGS
901ITLVTGMGIY ICLRKGEKHP REDENLEVQI PLKGKIDLHM RERKPMDISN I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

951 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.