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PCDH18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH18 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111483-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH18 (NM_019035), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1562 | PCDH18 | PCDH68L | Protocadherin 18 | Protocadherin 68-like protein | Protocadherin-18
Origin Species
Human
NCBI GenBank
AB046782
SwissProt
Q9HCL0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHQMNAKMHF RFVFALLIVS FNHDVLGKNL KYRIYEEQRV GSVIARLSED VADVLLKLPN
61PSTVRFRAMQ RGNSPLLVVN EDNGEISIGA TIDREQLCQK NLNCSIEFDV ITLPTEHLQL
121FHIEVEVLDI NDNSPQFSRS LIPIEISESA AVGTRIPLDS AFDPDVGENS LHTYSLSAND
181FFNIEVRTRT DGAKYAELIV VRELDRELKS SYELQLTASD MGVPQRSGSS ILKISISDSN
241DNSPAFEQQS YIIQLLENSP VGTLLLDLNA TDPDEGANGK IVYSFSSHVS PKIMETFKID
301SERGHLTLFK QVDYEITKSY EIDVQAQDLG PNSIPAHCKI IIKVVDVNDN KPEININLMS
361PGKEEISYIF EGDPIDTFVA LVRVQDKDSG LNGEIVCKLH GHGHFKLQKT YENNYLILTN
421ATLDREKRSE YSLTVIAEDR GTPSLSTVKH FTVQINDIND NPPHFQRSRY EFVISENNSP
481GAYITTVTAT DPDLGENGQV TYTILESFIL GSSITTYVTI DPSNGAIYAL RIFDHEEVSQ
541ITFVVEARDG GSPKQLVSNT TVVLTIIDEN DNVPVVIGPA LRNNTAEITI PKGAESGFHV
601TRIRAIDRDS GVNAELSCAI VAGNEENIFI IDPRSCDIHT NVSMDSVPYT EWELSVIIQD
661KGNPQLHTKV LLKCMIFEYA ESVTSTAMTS VSQASLDVSM IIIISLGAIC AVLLVIMVLF
721ATRCNREKKD TRSYNCRVAE STYQHHPKRP SRQIHKGDIT LVPTINGTLP IRSHHRSSPS
781SSPTLERGQM GSRQSHNSHQ SLNSLVTISS NHVPENFSLE LTHATPAVEQ VSQLLSMLHQ
841GQYQPRPSFR GNKYSRSYRY ALQDMDKFSL KDSGRGDSEA GDSDYDLGRD SPIDRLLGEG
901FSDLFLTDGR IPAAMRLCTE ECRVLGHSDQ CWMPPLPSPS SDYRSNMFIP GEEFPTQPQQ
961QHPHQSLEDD AQPADSGEKK KSFSTFGKDS PNDEDTGDTS TSSLLSEMSS VFQRLLPPSL
1021DTYSECSEVD RSNSLERRKG PLPAKTVGYP QGVAAWAAST HFQNPTTNCG PPLGTHSSVQ
1081PSSKWLPAME EIPENYEEDD FDNVLNHLND GKHELMDASE LVAEINKLLQ DVRQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1135 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.