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PCDH17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH17 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDH17 antibodies →
$504.00each
Catalog Number: LS-G120316-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH17 (NM_001040429), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH68 | Protocadherin 68 | Protocadherin 17 | PCDH17 | PCH68 | Protocadherin-17 | Protocadherin-68
Origin Species
Human
NCBI GenBank
AL445288
SwissProt
O14917

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.23 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYLSICCCFL LWAPALTLKN LNYSVPEEQG AGTVIGNIGR DARLQPGLPP AERGGGGRSK
61SGSYRVLENS APHLLDVDAD SGLLYTKQRI DRESLCRHNA KCQLSLEVFA NDKEICMIKV
121EIQDINDNAP SFSSDQIEMD ISENAAPGTR FPLTSAHDPD AGENGLRTYL LTRDDHGLFG
181LDVKSRGDGT KFPELVIQKA LDREQQNHHT LVLTALDGGE PPRSATVQIN VKVIDSNDNS
241PVFEAPSYLV ELPENAPLGT VVIDLNATDA DEGPNGEVLY SFSSYVPDRV RELFSIDPKT
301GLIRVKGNLD YEENGMLEID VQARDLGPNP IPAHCKVTVK LIDRNDNAPS IGFVSVRQGA
361LSEAAPPGTV IALVRVTDRD SGKNGQLQCR VLGGGGTGGG GGLGGPGGSV PFKLEENYDN
421FYTVVTDRPL DRETQDEYNV TIVARDGGSP PLNSTKSFAI KILDENDNPP RFTKGLYVLQ
481VHENNIPGEY LGSVLAQDPD LGQNGTVSYS ILPSHIGDVS IYTYVSVNPT NGAIYALRSF
541NFEQTKAFEF KVLAKDSGAP AHLESNATVR VTVLDVNDNA PVIVLPTLQN DTAELQVPRN
601AGLGYLVSTV RALDSDFGES GRLTYEIVDG NDDHLFEIDP SSGEIRTLHP FWEDVTPVVE
661LVVKVTDHGK PTLSAVAKLI IRSVSGSLPE GVPRVNGEQH HWDMSLPLIV TLSTISIILL
721AAMITIAVKC KRENKEIRTY NCRIAEYSHP QLGGGKGKKK KINKNDIMLV QSEVEERNAM
781NVMNVVSSPS LATSPMYFDY QTRLPLSSPR SEVMYLKPAS NNLTVPQGHA GCHTSFTGQG
841TNASETPATR MSIIQTDNFP AEPNYMGSRQ QFVQSSSTFK DPERASLRDS GHGDSDQADS
901DQDTNKGSCC DMSVREALKM KTTSTKSQPL EQEPEECVNC TDECRVLGHS DRCWMPQFPA
961ANQAENADYR TNLFVPTVEA NVETETYETV NPTGKKTFCT FGKDKREHTI LIANVKPYLK
1021AKRALSPLLQ EVPSASSSPT KACIEPCTST KGSLDGCEAK PGALAEASSQ YLPTDSQYLS
1081PSKQPRDPPF MASDQMARVF ADVHSRASRD SSEMGAVLEQ LDHPNRDLGR ESVDAEEVVR
1141EIDKLLQDCR GNDPVAVRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1159 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.