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PCDH10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCDH10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCDH10 antibodies →
$725.00each
Catalog Number: LS-G110971-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDH10 (NM_020815), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1400 | OL-protocadherin | OL-PCDH | Protocadherin-10 | Ortholog of OL-pcdh | PCDH10 | Protocadherin 10
Origin Species
Human
NCBI GenBank
AL157470
SwissProt
Q9P2E7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIVLLLFALL WMVEGVFSQL HYTVQEEQEH GTFVGNIAED LGLDITKLSA RGFQTVPNSR
61TPYLDLNLET GVLYVNEKID REQICKQSPS CVLHLEVFLE NPLELFQVEI EVLDINDNPP
121SFPEPDLTVE ISESATPGTR FPLESAFDPD VGTNSLRDYE ITPNSYFSLD VQTQGDGNRF
181AELVLEKPLD REQQAVHRYV LTAVDGGGGG GVGEGGGGGG GAGLPPQQQR TGTALLTIRV
241LDSNDNVPAF DQPVYTVSLP ENSPPGTLVI QLNATDPDEG QNGEVVYSFS SHISPRAREL
301FGLSPRTGRL EVSGELDYEE SPVYQVYVQA KDLGPNAVPA HCKVLVRVLD ANDNAPEISF
361STVKEAVSEG AAPGTVVALF SVTDRDSEEN GQVQCELLGD VPFRLKSSFK NYYTIVTEAP
421LDREAGDSYT LTVVARDRGE PALSTSKSIQ VQVSDVNDNA PRFSQPVYDV YVTENNVPGA
481YIYAVSATDR DEGANAQLAY SILECQIQGM SVFTYVSINS ENGYLYALRS FDYEQLKDFS
541FQVEARDAGS PQALAGNATV NILIVDQNDN APAIVAPLPG RNGTPAREVL PRSAEPGYLL
601TRVAAVDADD GENARLTYSI VRGNEMNLFR MDWRTGELRT ARRVPAKRDP QRPYELVIEV
661RDHGQPPLSS TATLVVQLVD GAVEPQGGGG SGGGGSGEHQ RPSRSGGGET SLDLTLILII
721ALGSVSFIFL LAMIVLAVRC QKEKKLNIYT CLASDCCLCC CCCGGGGSTC CGRQARARKK
781KLSKSDIMLV QSSNVPSNPA QVPIEESGGF GSHHHNQNYC YQVCLTPESA KTDLMFLKPC
841SPSRSTDTEH NPCGAIVTGY TDQQPDIISN GSILSNEVRL KRKDHHLSSP PSESLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

896 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.