Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PBXIP1 / HPIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111110-100
Catalog Number: LS-G111110
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PBXIP1 (NM_020524), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PBXIP1 | HPIP
- Origin Species
- Human
- NCBI GenBank
- NM_020524
- SwissProt
- Q96AQ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASCPDSDNS WVLAGSESLP VETLGPASRM DPESERALQA PHSPSKTDGK ELAGTMDGEG61TLFQTESPQS GSILTEETEV KGTLEGDVCG VEPPGPGDTV VQGDLQETTV VTGLGPDTQD121LEGQSPPQSL PSTPKAAWIR EEGRCSSSDD DTDVDMEGLR RRRGREAGPP QPMVPLAVEN181QAGGEGAGGE LGISLNMCLL GALVLLGLGV LLFSGGLSES ETGPMEEVER QVLPDPEVLE241AVGDRQDGLR EQLQAPVPPD SVPSLQNMGL LLDKLAKENQ DIRLLQAQLQ AQKEELQSLM301HQPKGLEEEN AQLRGALQQG EAFQRALESE LQQLRARLQG LEADCVRGPD GVCLSGGRGP361QGDKAIREQG PREQEPELSF LKQKEQLEAE AQALRQELER QRRLLGSVQQ DLERSLQDAS421RGDPAHAGLA ELGHRLAQKL QGLENWGQDP GVSANASKAW HQKSHFQNSR EWSGKEKWWD481GQRDRKAEHW KHKKEESGRE RKKNWGGQED REPAGRWKEG RPRVEESGSK KEGKRQGPKE541PPRKSGSFHS SGEKQKQPRW REGTKDSHDP LPSWAELLRP KYRAPQGCSG VDECARQEGL601TFFGTELAPV RQQELASLLR TYLARLPWAG QLTKELPLSP AFFGEDGIFR HDRLRFRDFV661DALEDSLEEV AVQQTGDDDE VDDFEDFIFS HFFGDKALKK RSGKKDKHSQ SPRAAGPREG721HSHSHHHHHR GTRTRPLEQKLISEEDLAANDILDYKDDDDKV
731 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.